[Objectives]This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization (SSH) in the testis of male tilapia under the stress of methomyl by using SSH technology.[Method...[Objectives]This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization (SSH) in the testis of male tilapia under the stress of methomyl by using SSH technology.[Methods]Using male tilapia as the test animal,the forward and reserve libraries of SSH in the testis of tilapia under the stress of methomyl were constructed by using the SSH technology.[Results]45 expressed sequence tags (ESTs) were obtained,and 25 expressed sequence tags were successfully noted,including 13 forward libraries and 12 reverse libraries.The genes with confirmed functions were classified into five types.Genes related to catalytic activity and cell characteristics were up-regulated,while genes related to structural molecule's activity and biological process were down-regulated.The expression amount of integrin β1 was up-regulated,while serine/threonine protein kinase pim-3,Ca^2+-ATPase,Na^+-K^+-ATPase and ribosomal protein L22 were down-regulated.[Conclusions]The research results could lay a foundation for revealing the molecular mechanism of methomyl's reproductive toxicity to tilapia.展开更多
Guangxi Zhuang Autonomous Region is an excellent area for tilapia breeding in China,and the development of tilapia industry has received high attention of both the state and Guangxi government. The foreign exchange ea...Guangxi Zhuang Autonomous Region is an excellent area for tilapia breeding in China,and the development of tilapia industry has received high attention of both the state and Guangxi government. The foreign exchange earning is an essential industrial form of tilapia industry in Guangxi. Thus,enhancing the competitiveness of export-oriented tilapia processing is of great significance to the development of tilapia industry. On the basis of field survey,this paper analyzed current situation of export-oriented tilapia processing in Guangxi. At present,tilapia processing industry in Guangxi has realized preliminary standardization,large-scale and grouping,but it is still faced with some problems and challenges,such as low industrialization level,no balanced marketing,and imperfect product quality safety monitoring measures. Therefore,it is required to create favorable environment for export-oriented tilapia processing industry in Guangxi through enhancing policy support,increasing financial input and supporting tilapia processing leading enterprises.展开更多
This study was conducted to find the differentially expressed genes and understand the transcriptome change characteristics in the testis of tilapia(Oreochromis niloticus) stressed by methomyl.The tilapia were exposed...This study was conducted to find the differentially expressed genes and understand the transcriptome change characteristics in the testis of tilapia(Oreochromis niloticus) stressed by methomyl.The tilapia were exposed to 200 μg/L methomyl for 30 d,and then the testis samples were collected and used for the highthroughput sequencing.The results showed that 369 significant differentially expressed genes were obtained,including 160 significantly down-regulated genes,and209 significantly up-regulated genes.The enrichment of the differentially expressed genes in ECM-receptor interaction signaling pathway and MAPK signaling pathway was obvious.The adhesion of testis tissue cells was influenced by methomyl stress,which induced the expression of related gene in ECM-receptor interaction up-regulating,such as laminins,integrin α6 and integrin β1,in order to stabilize testis tissue structure.JNK,MAP3 K and MKK4 in the JNK pathway were significantly up-regulated,which enhanced transcription factor AP-1 to promote the expression of P53,Bax and other pro-apoptotic proteins.The expression of growth-related factors in the ERK pathway of MAPK signal pathway was influenced by methomyl stress.The apoptosis-related genes and pregnancy-related genes have significant differential expression under methomyl stress,and thereby,the proliferation of reproduction cells in the testis tissue may be affected.展开更多
基金Supported by Basic Scientific Research Fund for Chinese Academy of Fishery Sciences(2015C02XK01)Youth Natural Scientific Foundation of Jiansu Province(BK20150117)Earmarked Fund for China Agriculture Research System(CARS-46)
文摘[Objectives]This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization (SSH) in the testis of male tilapia under the stress of methomyl by using SSH technology.[Methods]Using male tilapia as the test animal,the forward and reserve libraries of SSH in the testis of tilapia under the stress of methomyl were constructed by using the SSH technology.[Results]45 expressed sequence tags (ESTs) were obtained,and 25 expressed sequence tags were successfully noted,including 13 forward libraries and 12 reverse libraries.The genes with confirmed functions were classified into five types.Genes related to catalytic activity and cell characteristics were up-regulated,while genes related to structural molecule's activity and biological process were down-regulated.The expression amount of integrin β1 was up-regulated,while serine/threonine protein kinase pim-3,Ca^2+-ATPase,Na^+-K^+-ATPase and ribosomal protein L22 were down-regulated.[Conclusions]The research results could lay a foundation for revealing the molecular mechanism of methomyl's reproductive toxicity to tilapia.
基金Supported by Special Project of Modern Agricultural Industry Technical System Construction(CARS-49)
文摘Guangxi Zhuang Autonomous Region is an excellent area for tilapia breeding in China,and the development of tilapia industry has received high attention of both the state and Guangxi government. The foreign exchange earning is an essential industrial form of tilapia industry in Guangxi. Thus,enhancing the competitiveness of export-oriented tilapia processing is of great significance to the development of tilapia industry. On the basis of field survey,this paper analyzed current situation of export-oriented tilapia processing in Guangxi. At present,tilapia processing industry in Guangxi has realized preliminary standardization,large-scale and grouping,but it is still faced with some problems and challenges,such as low industrialization level,no balanced marketing,and imperfect product quality safety monitoring measures. Therefore,it is required to create favorable environment for export-oriented tilapia processing industry in Guangxi through enhancing policy support,increasing financial input and supporting tilapia processing leading enterprises.
基金Supported by Fundamental Research Fund of Freshwater Fisheries Research Center of Chinese Academy of Fishery Science(2017JBFM07)Natural Science Foundation of Jiangsu Province(BK20150117)Earmarked Fund for Modern Agro-industry Technology Research System(CARS-46)。
文摘This study was conducted to find the differentially expressed genes and understand the transcriptome change characteristics in the testis of tilapia(Oreochromis niloticus) stressed by methomyl.The tilapia were exposed to 200 μg/L methomyl for 30 d,and then the testis samples were collected and used for the highthroughput sequencing.The results showed that 369 significant differentially expressed genes were obtained,including 160 significantly down-regulated genes,and209 significantly up-regulated genes.The enrichment of the differentially expressed genes in ECM-receptor interaction signaling pathway and MAPK signaling pathway was obvious.The adhesion of testis tissue cells was influenced by methomyl stress,which induced the expression of related gene in ECM-receptor interaction up-regulating,such as laminins,integrin α6 and integrin β1,in order to stabilize testis tissue structure.JNK,MAP3 K and MKK4 in the JNK pathway were significantly up-regulated,which enhanced transcription factor AP-1 to promote the expression of P53,Bax and other pro-apoptotic proteins.The expression of growth-related factors in the ERK pathway of MAPK signal pathway was influenced by methomyl stress.The apoptosis-related genes and pregnancy-related genes have significant differential expression under methomyl stress,and thereby,the proliferation of reproduction cells in the testis tissue may be affected.
