Pan-genome and transcriptome analyses provide insights into genomic variation and differential gene expression profiles related to disease resistance and fatty acid biosynthesis in eastern black walnut (Juglans nigra)

Walnut(Juglans)species are used as nut crops worldwide.Eastern black walnut(EBW,Juglans nigra),a diploid,horticultural important woody species is native to much of eastern North America.Although it is highly valued for its wood and nut,there are few resources for understanding EBW genetics.Here,we present a high-quality genome assembly of J.nigra based on Illumina,Pacbio,and HiC technologies.The genome size was 540.8 Mb,with a scaffold N50 size of 35.1 Mb,and 99.0%of the assembly was anchored to 16 chromosomes.Using this genome as a reference,the resequencing of 74 accessions revealed the effective population size of J.nigra declined during the glacial maximum.A single whole-genome duplication event was identified in the J.nigra genome.Large syntenic blocks among J.nigra,Juglans regia,and Juglans microcarpa predominated,but inversions of more than 600 kb were identified.By comparing the EBW genome with those of J.regia and J.microcarpa,we detected InDel sizes of 34.9 Mb in J.regia and 18.3 Mb in J.microcarpa,respectively.Transcriptomic analysis of differentially expressed genes identified five presumed NBS-LRR(NUCLEOTIDE BINDING SITELEUCINE-RICH REPEAT)genes were upregulated during the development of walnut husks and shells compared to developing embryos.We also identified candidate genes with essential roles in seed oil synthesis,including FAD(FATTY ACID DESATURASE)and OLE(OLEOSIN).Our work advances the understanding of fatty acid bioaccumulation and disease resistance in nut crops,and also provides an essential resource for conducting genomics-enabled breeding in walnut.

Seasonal distribution of increased precipitation in maternal environments influences offspring performance of Potentilla tanacetifolia in a temperate steppe ecosystem

Aims Precipitation is predicted to increase in arid and semiarid regions under climate change,with greater changes in intra-and interannual distribution in the future.As a major limiting factor in these regions,changes in precipitation undoubtedly influence plant growth and productivity.However,how the temporal shifts in pre-cipitation will impact plant populations are uncertain.Methods A 3-year field experiment and a greenhouse experiment were conducted in a temperate grassland in northern China to examine the impacts of seasonal(spring and summer)increased precipitation on offspring performance of a common species,Potentilla tanacetifolia.Important Findings Our results showed that the amounts and timing of increased precipitation both played important roles in regulating offspring performance of P.tanacetifolia in the temperate steppe ecosystem.Increased precipitation in spring at maternal stage stimulated seed production,germination percentage and seedling biomass,whereas increased precipitation in summer at maternal stage stimulated seedling biomass.The timing of increased precipitation influenced seed attributes,whereas the amount of increased precipitation influenced offspring seedling biomass.Our results indicate that population development of P.tanacetifolia may be underestimated under future increased precipitation regime,if the transgenerational effect is not taken into account.