Ticks are well known as vectors of many viruses which usually do great harm to human and animal health.Yunnan Province,widely covered by flourishing vegetation and mainly relying on farming husbandry,is abundant with ...Ticks are well known as vectors of many viruses which usually do great harm to human and animal health.Yunnan Province,widely covered by flourishing vegetation and mainly relying on farming husbandry,is abundant with Rhipicephalus microplus ticks.Therefore,it is of great significance to characterize the viral profile present in R.microplus parasitizing on cattle in Yunnan Province.In this study,a total of 7387 R.microplus ticks were collected from cattle and buffalo in the northwest and southeast areas of Yunnan Province from 2015 to 2017.We investigated the virome of R.microplus using next-generation sequencing(NGS)and the prevalence of important identified viruses among tick groups by RT-PCR.It revealed the presence of diverse virus concerning chu-,rhabdo-,phlebo-,flavi-and parvo-viruses in Yunnan.These viruses consist of single-stranded,circular and segmented sense RNAs,showing a greatly diversity in genomic organization.Furthermore,continuous epidemiological survey among ticks reveals broad prevalence of three viruses(Yunnan mivirus 1,Wuhan tick vrius 1 and YN tick-associated phlebovirus 1)and two possible prevalent viruses including a flavivirus-like segmented virus(Jingmen tick virus)and a bovine hokovirus 2 in Yunnan.Serological investigation among cattle indicates that these identified viruses may be infectious to cattle and can elicit corresponding antibody.Our findings on R.microplus-associated viral community will contribute to the prevention of viral disease and tracking the viral evolution.Further analysis is needed to better elucidate the pathogenicity and natural circulation of these viruses.展开更多
What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces ar...What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces are traditionally endemic areas.What is added by this report?This is the first published outbreak of occupational brucellosis involving multiple clusters in Hubei Province.This investigation characterizes the transmission chain of the outbreak and reveals that provinces south of the Yangtze River are faced with a series of challenges and hurdles to overcome including,but not limited to,health education,law enforcement,and occupational protection.What are the implications for public health practice?In addition to measures aimed at this outbreak,the local agricultural department has issued special notices based on this investigation to adjust and strengthen local eradication program of brucellosis.展开更多
基金This work was jointly funded by the Scientific and Technological Basis Special Project grant(2013FY113500)from the Ministry of Science and Technology of Chinathe National Natural Science Foundation of China(81874274 and 81660558)+1 种基金the National Science and Technology Major Project on Important Infectious Diseases Prevention and Control(2018ZX10734-404)the Yunnan Health Training Project of High Level Talents(L-2017027).
文摘Ticks are well known as vectors of many viruses which usually do great harm to human and animal health.Yunnan Province,widely covered by flourishing vegetation and mainly relying on farming husbandry,is abundant with Rhipicephalus microplus ticks.Therefore,it is of great significance to characterize the viral profile present in R.microplus parasitizing on cattle in Yunnan Province.In this study,a total of 7387 R.microplus ticks were collected from cattle and buffalo in the northwest and southeast areas of Yunnan Province from 2015 to 2017.We investigated the virome of R.microplus using next-generation sequencing(NGS)and the prevalence of important identified viruses among tick groups by RT-PCR.It revealed the presence of diverse virus concerning chu-,rhabdo-,phlebo-,flavi-and parvo-viruses in Yunnan.These viruses consist of single-stranded,circular and segmented sense RNAs,showing a greatly diversity in genomic organization.Furthermore,continuous epidemiological survey among ticks reveals broad prevalence of three viruses(Yunnan mivirus 1,Wuhan tick vrius 1 and YN tick-associated phlebovirus 1)and two possible prevalent viruses including a flavivirus-like segmented virus(Jingmen tick virus)and a bovine hokovirus 2 in Yunnan.Serological investigation among cattle indicates that these identified viruses may be infectious to cattle and can elicit corresponding antibody.Our findings on R.microplus-associated viral community will contribute to the prevention of viral disease and tracking the viral evolution.Further analysis is needed to better elucidate the pathogenicity and natural circulation of these viruses.
文摘What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces are traditionally endemic areas.What is added by this report?This is the first published outbreak of occupational brucellosis involving multiple clusters in Hubei Province.This investigation characterizes the transmission chain of the outbreak and reveals that provinces south of the Yangtze River are faced with a series of challenges and hurdles to overcome including,but not limited to,health education,law enforcement,and occupational protection.What are the implications for public health practice?In addition to measures aimed at this outbreak,the local agricultural department has issued special notices based on this investigation to adjust and strengthen local eradication program of brucellosis.
