Previous studies have indicated that two monoclonal antibodies(mAbs;A1-10 and H1-84)of the hemagglutinin(HA)antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are h...Previous studies have indicated that two monoclonal antibodies(mAbs;A1-10 and H1-84)of the hemagglutinin(HA)antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue(Guo et al.in Immunobiology 220:941-946,2015).However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed.In the present study,the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software.The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software.Key amino acids and variable sequences of the antibodies were identified using abYsis software.The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs.One of the peptides,P2(LVLWGIHHP191-199),bound both of the mAbs and was located in the head region of HA.The key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described.A heterophilic epitope on H1N1 influenza virus HA was also introduced.The existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection,which might aid in influenza prevention and control.展开更多
What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces ar...What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces are traditionally endemic areas.What is added by this report?This is the first published outbreak of occupational brucellosis involving multiple clusters in Hubei Province.This investigation characterizes the transmission chain of the outbreak and reveals that provinces south of the Yangtze River are faced with a series of challenges and hurdles to overcome including,but not limited to,health education,law enforcement,and occupational protection.What are the implications for public health practice?In addition to measures aimed at this outbreak,the local agricultural department has issued special notices based on this investigation to adjust and strengthen local eradication program of brucellosis.展开更多
基金supported by The National Key Research and Development Program of China (Grant No. 2016YFD0500700)The Natural Science Basic Research Program of Shaanxi Province (Grant No. 2016JM8065)Shaanxi Provincial People’s Hospital Incubation Fund Program (Grant No. 2015YX-4)
文摘Previous studies have indicated that two monoclonal antibodies(mAbs;A1-10 and H1-84)of the hemagglutinin(HA)antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue(Guo et al.in Immunobiology 220:941-946,2015).However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed.In the present study,the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software.The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software.Key amino acids and variable sequences of the antibodies were identified using abYsis software.The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs.One of the peptides,P2(LVLWGIHHP191-199),bound both of the mAbs and was located in the head region of HA.The key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described.A heterophilic epitope on H1N1 influenza virus HA was also introduced.The existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection,which might aid in influenza prevention and control.
文摘What is already known on this topic?Human brucellosis,a neglected zoonotic disease,causes more than 500,000 new cases each year globally.The disease is of major public health concern in China,and northern provinces are traditionally endemic areas.What is added by this report?This is the first published outbreak of occupational brucellosis involving multiple clusters in Hubei Province.This investigation characterizes the transmission chain of the outbreak and reveals that provinces south of the Yangtze River are faced with a series of challenges and hurdles to overcome including,but not limited to,health education,law enforcement,and occupational protection.What are the implications for public health practice?In addition to measures aimed at this outbreak,the local agricultural department has issued special notices based on this investigation to adjust and strengthen local eradication program of brucellosis.
