FGS, isolated from the water solution of enzymolyzed laminaria japonica, is a mixture of acid heteroglycans. Four fractions F1, F2, F3, and F4 were obtained from FGS by ion exchange chromatography. After further purif...FGS, isolated from the water solution of enzymolyzed laminaria japonica, is a mixture of acid heteroglycans. Four fractions F1, F2, F3, and F4 were obtained from FGS by ion exchange chromatography. After further purified by gel filtration chromatography on a sepharose 2B and 6B column, we obtained F9, F10, F11, and F12. They showed single band when identified by electrophoresis. The molecular weight of F9, F10, F11, and F12 was estimated to be 216, 120, 138 and 140KD respectively. They containedа-glucosidic bond by IR and 1H-NMR analysis. The typical absorption peaks of these polysaccharides were showed in UV and IR spectra. These polysaccharides contained rha, fuc, man, gal, and uronate when identified by paper chromatography (p.c) and gas chromatography (GC). The molar ratio of these sugars was also assayed.展开更多
The immunomodulating effects of F12 on mouse peritoneal macrophages was intended to be studied with different doses of 20, 40, 80 and 120mg/Kg. F12 increased peroxidase activity in dexc dependent mammer from 0.02±...The immunomodulating effects of F12 on mouse peritoneal macrophages was intended to be studied with different doses of 20, 40, 80 and 120mg/Kg. F12 increased peroxidase activity in dexc dependent mammer from 0.02±0.001 to 0.175±0.038, 0.211±0.041, 0.137±0.045 and 0.078±0.036, respectively, increased cytotoxicity from 127.33±22.96 to 162.74±19.53, 237.30±25.15 , 178.62±36.22 and 158.66±42.75dpm, respectively, promoted phagocytic activity from 0.03±0.01 to0.21±0.016, 0.43±0.041, 0.40±0.032 and 0.30±0.160b. Furthermore, F12 in a concentration of 15, 30,60,120μg/ml enhanced IL-1 production from 0.15±0.02 to 0.20±0.005b 0.21±0.02,0.22±0.005,0.24±0.002 and 0.22±0.02,respectively. Thus F12 can be considered as an effective stimulator of macrophages.展开更多
文摘FGS, isolated from the water solution of enzymolyzed laminaria japonica, is a mixture of acid heteroglycans. Four fractions F1, F2, F3, and F4 were obtained from FGS by ion exchange chromatography. After further purified by gel filtration chromatography on a sepharose 2B and 6B column, we obtained F9, F10, F11, and F12. They showed single band when identified by electrophoresis. The molecular weight of F9, F10, F11, and F12 was estimated to be 216, 120, 138 and 140KD respectively. They containedа-glucosidic bond by IR and 1H-NMR analysis. The typical absorption peaks of these polysaccharides were showed in UV and IR spectra. These polysaccharides contained rha, fuc, man, gal, and uronate when identified by paper chromatography (p.c) and gas chromatography (GC). The molar ratio of these sugars was also assayed.
基金The National 95 Key-Attacking Studies (96-C01-05-02)
文摘The immunomodulating effects of F12 on mouse peritoneal macrophages was intended to be studied with different doses of 20, 40, 80 and 120mg/Kg. F12 increased peroxidase activity in dexc dependent mammer from 0.02±0.001 to 0.175±0.038, 0.211±0.041, 0.137±0.045 and 0.078±0.036, respectively, increased cytotoxicity from 127.33±22.96 to 162.74±19.53, 237.30±25.15 , 178.62±36.22 and 158.66±42.75dpm, respectively, promoted phagocytic activity from 0.03±0.01 to0.21±0.016, 0.43±0.041, 0.40±0.032 and 0.30±0.160b. Furthermore, F12 in a concentration of 15, 30,60,120μg/ml enhanced IL-1 production from 0.15±0.02 to 0.20±0.005b 0.21±0.02,0.22±0.005,0.24±0.002 and 0.22±0.02,respectively. Thus F12 can be considered as an effective stimulator of macrophages.
