MELLT3 protects against cerebral ischemia-reperfusion (I/R) injury through up-regulation of m6A modification

Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury(CIRI).The exact mechanisms underlying I/R injury are unclear.In this study,we aimed to determine the role of m6A-modified methylase complex methyltransferase-like 3(METTL3)in cerebral ischemiareperfusion(I/R)injury.We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytescerebellar(MA-C)and the brain of middle cerebral artery occlusion(MCAO)model mice.METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation,which increased with METTL3 over-expression.Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis,which was significantly reduced with METTL3 up-regulation.In an MCAO model,METTL3 overexpression significantly reduced cerebral infarction area and decreased brain cell apoptosis,indicating that METTL3 OE treatment could ameliorate brain edema and injury.Thus,METTL3 could be used as a target to treat I/R injury.

Clinical observation on the effect of electroacupuncture treatment for chloasma in young and middle-aged women

Objective:To evaluate the clinical effect of electroacupuncture treatment for chloasma in young and middle-aged women.Methods:Sixty young and middle-aged female patients with chloasma were divided into treatment group(n=30)and control group(n=30)according to random number table.In the treatment group,the electroacupuncture was applied to the facial acupoints and"Vagus"in the cavity of concha in the ear.The selection of facial acupoints was relating to the running course of facial nerve trunk:Yifeng(翳风TE17),Shangguan(上关GB3)-Sizhukong(丝竹空TE 23)(Temporal branch),Xiaguan(下关ST7)-Sibai(四白ST2)(Zygomatic branch),Qianzheng(牵正Extra)-Quanliao(颧髎SI18)(Zygomatic branch),Jiache(颊车ST6)-Jiachengjiang(夹承浆Extra)(Buccal branch),Hegu(合谷LI 4).Electro-stimulation was adopted at"Vagus"in the cavity of concha in the ear(two distribution points of the vagus in the cavity of concha of each ear).The control group received conventional acupuncture(surrounding method of needling at the Ashi points in the facial lesion area and 15 acupoints which were selected in the highest frequency in the conventional acupuncture treatment).After 8 weeks of treatment,the clinical effects were compared.The total score of facial skin lesion,score of skin lesion color,score of lesion area,and effective rate of treatment for chloasma were used to evaluate the clinical effect of the two groups before and after treatment.Results:After treatment,the effective rate of the treatment group was 95.00%,that of the control group was 67.50%.The effect of the treatment group was better than that of the control group,and the difference was statistically signifiicant(x2=9.928,P=0.002).Before treatment,there was no difference in the area score,color score,and total score of skin lesions between the two groups(P>0.05).After treatment,the area score,color score,and total score of skin lesions in the treatment group were(1.05±0.64),(1.05±0.64),and(2.10±0.98)points respectively,while those in the control group were(1.38±0.63),(1.28±0.75),and(2.65±1.19)points respectively.The area score,color score,and total score of skin lesions in the treatment group were lower than those in the control group,with statistically significant differences(all P<0.05).Conclusion:electroacupuncture is more effective than conventional acupuncture in treating chloasma in young and middle-aged women.

Electroacupuncture for postpartum urinary retention:A randomized controlled study

Objective:To observe the clinical effect of electroacupuncture on postpartum urinary retention(PPUR)through a randomized controlled study.Methods:Seventy-two PPUR puerperae who met the inclusion criteria were divided into the observation group(electroacupuncture at Ciliao(次髎BL 32) and Zhongliao(中髎 BL 33) group) and the control group(sham acupuncture group) according to the random number table method,with 36 cases in each.Urine catheters were retained.After 3 days of treatment,the therapeutic effect of PPUR was compared between the two groups.Results:In the observation group,the first urination time was significantly earlier than that in the control group,the first urination volume was significantly more than that in the control group,the bladder residual urine volume was significantly less than that in the control group,and the urinary infection was significantly less than that in the control group,the differences were statistically significant(all P <0.001).Compared with the control group,there were statistically significant differences in catheter removal,independent urination,cured cases,effective cases,ineffective cases,and total effective cases in the observation group(all P <0.05).Conclusion:The therapeutic value of electroacupuncture at BL32 and BL33 for primiparae with PPUR is significantly obvious.This therapy is worthy of clinical promotion.