Ever since its outbreak,the COVID-19 pandemic caused by SARS-CoV-2 has been spreading rapidly causing a global health crisis.Accurate diagnosis and instituting appropriate intervention to relevant individuals are esse...Ever since its outbreak,the COVID-19 pandemic caused by SARS-CoV-2 has been spreading rapidly causing a global health crisis.Accurate diagnosis and instituting appropriate intervention to relevant individuals are essential not only to slow down the spread of this pandemic but also to manage our resources efficiently.To date,testing for SARS-CoV-2 infection mostly relies on reverse transcription-quantitative polymerase chain reaction(RT-qPCR)on a nasopharyngeal or saliva specimen and remains the gold standard for diagnosis as it exhibits high sensitivity and specificity.展开更多
Invasive infection caused by Streptococcus pyogenes emm89 strains has been increasing in several countries linked to a recently emergent clade of emm89 strains,designated clade 3.In Japan,the features of emm89 S.pyoge...Invasive infection caused by Streptococcus pyogenes emm89 strains has been increasing in several countries linked to a recently emergent clade of emm89 strains,designated clade 3.In Japan,the features of emm89 S.pyogenes strains,such as clade classification,remains unknown.In this study,we collected emm89 strains isolated from both streptococcal toxic shock syndrome(STSS)(89 STSS isolates)and noninvasive infections(72 non-STSS isolates)in Japan from 2011 to 2019,and conducted wholegenome sequencing and comparative analysis,which resulted in classification of a large majority into clade 3 regardless of disease severity.In addition,invasive disease-associated factors were found among emm89 strains,including mutations of control of virulence sensor,and absence of the hylP1 gene encoding hyaluronidase.These findings provide new insights into genetic features of emm89 strains.展开更多
基金supported in port by Japan Agency for Medical Research and Development(AMED,IP19fk0108110 and JP20he0522001)by Health Labour Sciences research grant from the Ministry of Health Labour and Welfare(19HA1003)。
文摘Ever since its outbreak,the COVID-19 pandemic caused by SARS-CoV-2 has been spreading rapidly causing a global health crisis.Accurate diagnosis and instituting appropriate intervention to relevant individuals are essential not only to slow down the spread of this pandemic but also to manage our resources efficiently.To date,testing for SARS-CoV-2 infection mostly relies on reverse transcription-quantitative polymerase chain reaction(RT-qPCR)on a nasopharyngeal or saliva specimen and remains the gold standard for diagnosis as it exhibits high sensitivity and specificity.
基金AMED(Grant numbers:JP19fk0108044,JP19fm0208007)Japanese Society for the Promotion of Science(JSPS)KAKENHI(Grant numbers:19H03825,20K18474)。
文摘Invasive infection caused by Streptococcus pyogenes emm89 strains has been increasing in several countries linked to a recently emergent clade of emm89 strains,designated clade 3.In Japan,the features of emm89 S.pyogenes strains,such as clade classification,remains unknown.In this study,we collected emm89 strains isolated from both streptococcal toxic shock syndrome(STSS)(89 STSS isolates)and noninvasive infections(72 non-STSS isolates)in Japan from 2011 to 2019,and conducted wholegenome sequencing and comparative analysis,which resulted in classification of a large majority into clade 3 regardless of disease severity.In addition,invasive disease-associated factors were found among emm89 strains,including mutations of control of virulence sensor,and absence of the hylP1 gene encoding hyaluronidase.These findings provide new insights into genetic features of emm89 strains.
