Prediction of the mechanism of prostate prescription Ⅰ in the treatment of chronic prostatitis based on integrative pharmacology of traditional Chinese medicine

Objective:To investigate potential mechanism of Prostate prescriptionⅠin the treatment of chronic prostatitis based on integrated pharmacology.Methods:with the help of TCMIP,the effective compounds of Prostate prescriptionⅠwere screened,and the potential target database of Prostate prescriptionⅠwas established by using the target prediction function of TCMIP.Based on the Human Phenotype Ontology database and protein interaction network(PPI)database,the disease target of CP was identified and the target interaction network was constructed;Through the GO function analysis and KEGG pathway enrichment analysis of the candidate targets,combined with Chinese and foreign literature studies to predict the potential Signaling pathway of Prostate prescriptionⅠin the treatment of CP.Results:79 effective compounds of Prostate prescriptionⅠexerted its effect on 33 targets through 11 pathways.Conclusion:Clinical efficacy of Prostate prescriptionⅠon CP may be reflected in antiinflammation and anti-tumor,cell metabolism,immune regulation,neuroregulation and so on.

Benidipine-loaded nanoflower-likemagnesium silicate improves bone regeneration

Regeneration and reconstruction of bone tissue is always a challenge for clinicians due to the uncertainty of bone repair materials in terms of long-term and efficient effects on osteoblasts.Here,we propose a novel strategy combining benidipine,an antihypertensive drug and nanoparticles to synergistically promote the healing of bone defects.Loose and porous benidipine-loaded magnesium silicate nanoparticles were prepared and validated for their biosafety.The nanoparticles were efficiently taken up by preosteoblasts and uniformly distributed around the nucleus.After internalization into cells,the nanosystem is degraded by lysosomes,and the effect of promoting osteogenic differentiation is reflected by the continuous release of benidipine,silicon and magnesium ions.Our results clearly evaluated that the nanoflower-like magnesium silicate delivering benidipine tends to be more appropriate for the bone regeneration in preosteoblasts,indicating that it might be a potential approach in guiding bone repair in clinical applications.

Effect of acupuncture intervention on the intestinal mucosal inflammatory response and immune response balance in animals with ulcerative colitis

Objective:To study the effect of acupuncture intervention on the intestinal mucosal inflammatory response and immune response balance in animals with ulcerative colitis (UC).Methods:Adult, male SPF SD rats were selected and randomly divided into the control group, UC group and acupuncture group, and then the acupuncture intervention was established after the UC animal model was established. 14 d after intervention, the expression of inflammatory mediators and Th1/Th2/Th17/Treg cytokines in intestinal mucosa, and the levels of inflammatory mediators and Th1/Th2/Th17/Treg cytokines in serum were detected. Results:NF-kB, HMGB-1, TNF-α, IL-1β, IFN-γ and IL-17 mRNA expression in intestinal mucosa as well as HMGB-1, TNF-α, IL-1β, IFN-γ and IL-17 levels in serum of UC group were significantly higher than those of control group while IL-4, IL-5 and TGF-β1 mRNA expression in intestinal mucosa as well as IL-4, IL-5 and TGF-β1 levels in serum were significantly lower than those of control group;NF-kB, HMGB-1, TNF-α, IL-1β, IFN-γ and IL-17 mRNA expression in intestinal mucosa as well as HMGB-1, TNF-α, IL-1β, IFN-γ and IL-17 levels in serum of acupuncture group were significantly lower than those of UC group while IL-4, IL-5 and TGF-β1 mRNA expression in intestinal mucosa as well as IL-4, IL-5 and TGF-β1 levels in serum were significantly higher than those of UC group. Conclusions: Acupuncture intervention can regulate the intestinal mucosal inflammatory response and immune response of animals with ulcerative colitis.

Adenovirus-mediated NDRG2 inhibits the proliferation of human renal cell carcinoma cell line OS-RC-2 in vitro

Objective:To investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of human renal cell carcinoma cell line OS-RC-2 in vitro.Methods:NDRG2 was harvested by RT-PCR,confirmed by DNA sequencing,and then cloned into the eukaryotic expression vector pIRES2-EGFP,which encodes green fluorescent protein(GFP),to construct p1RES2-EGFP-NDRG2 plasmid.OS-RC-2 cells with NDRG2 negative expression were transfected with p1RES2-EGFP-NDRG2 plasmid.The growth of transfected OS-RC-2 cells was observed under light and fluorescence microscopes.After colony-forming cell assays,cell proliferation detection and MTT assays,the growth curves of cells in each group were plotted to investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of OS-RC-2 cells.Cell cycle was determined by flow cytometry.Confocal laser scanning microscopy showed that NDRG2 protein was specifically located on subcellular organelle.Results:A eukaryotic expression vector p1RES2-EGFP-NDRG2 was successfully constructed.After NDRG2 transfection,the growth of OS-RC-2 cells was inhibited.Flow cytometry showed that cells were arrested in S phase but the peak of cell apoptosis was not present,and confocal laser scanning microscopy showed that NDRG2 protein was located in mitochondrion.Conclusions:NDRG2 can significantly inhibit the proliferation of OS-RC-2 cells in vitro and its protein is specifically expressed in the mitochondrion.

Effective response to crizotinib of concurrent KIF5B-MET and MET-CDR2-rearranged non-small cell lung cancer:A case report

BACKGROUND Due to the rarity of mesenchymal-epithelial transition factor(MET)fusions,the clinical efficacy of crizotinib has only been described in a few patients with MET fusions involving various fusion partners.Herein,we report the clinical response to crizotinib of a patient with advanced poorly differentiated non-small cell carcinoma(NSCLC)having concurrent MET fusions.CASE SUMMARY A 46-year-old woman was diagnosed with poorly differentiated NSCLC(T4 N3 M1).With no classic driver mutations,she was treated with two cycles of gemcitabine and cisplatin without clinical benefit.Targeted sequencing revealed the detection of two concurrent MET fusions,KIF5 B-MET and novel MET-CDR2.Crizotinib was initiated at a dose of 250 mg twice daily.Within 4 wk of crizotinib therapy,repeat computed chromatography revealed a dramatic reduction in primary and metastatic lesions,assessed as partial response.She continued to benefit from crizotinib for 3 mo until disease progression and died within 1 mo despite receiving nivolumab therapy.CONCLUSION Crizotinib sensitivity was observed in an advanced poorly differentiated NSCLC patient with concurrent MET fusions KIF5 B-MET and MET-CDR2.Crizotinib can serve as a therapeutic option for patients with MET fusions.In addition,our case also highlights the importance of comprehensive genomic profiling particularly in patients with no classic driver mutation for guiding alternative therapeutic decisions.

Effect of Chaishao Chengqi Decoction on inflammation and prognosis of moderate and severe acute pancreatitis of heat stagnation and Fu-organ excess type

Objective:To explore the curative effect of Chaishao Chengqi Decoction on moderate to severe acute pancreatitis of heat stagnation and Fu-organ excess type and its effect on inflammatory reaction,and to provide new ideas for clinical treatment and prognosis evaluation.Methods:60 patients with moderate to severe acute pancreatitis of heat stagnation and Fu-organ excess type from June 2017 to June 2019 were randomly divided into control group treated with conventional therapy and treatment group treated with Chaishao Chengqi Decoction for 2 weeks.The differences of clinical efficacy and TCM syndromes between the two groups were compared.Ranson score,Balthazar CTSI score,SIRS score and modified Marshall score were recorded before and after treatment.The levels of inflammatory factors and oxidative stress-related molecules were compared before and after treatment.Result:After treatment,the total effective rates of the control group and the treatment group were 80.0%and 93.3%,respectively,with statistical difference(χ2=8.845,P=0.027);after treatment,the total effective rates of TCM syndromes evaluation of the control group and the treatment group were 76.7%and 93.3%,respectively,with statistical difference(χ2=10.024,P=0.012);after treatment,the Ranson score,Balthazar CTSI score,SIRS score and improved Marshall score were all significantly decreased(P<0.05),and the decrease was more significant in the treatment group(P<0.05);after treatment,the serum levels of TNF-α,IL-6 and MDA were significantly decreased in both groups,and superoxide dismutase(SOD)was significantly increased(P<0.05),and the treatment group improved more(P<0.05).Conclusion:Chaishao Chengqi Decoction has a good curative effect on moderate to severe acute pancreatitis of heat stagnation and Fu-organ excess type,and can effectively improve inflammatory response and oxidative stress,which has a high clinical significance.

空气波压力治疗仪治疗奥沙利铂神经毒性的临床观察(英文)

Objective: The aim of this study was to observe the efficacy of air wave pressure therapeutic equipment in prevention of oxaliplatin-inducted neurotoxicity. Methods: Forty-five patients with colorectal cancer were randomly divided into treatment group and control group, treatment group were given the treatment of air wave pressure therapeutic equipment during chemotherapy with oxaliplatin, the control group were given preventive treatment, the oxaliplatin-inducted neurotoxicity was evaluated after each cycle of chemotherapy. Evaluate the chemotherapy efficacy after the third cycle and sixth cycle of chemotherapy. Results: The treatment group have lower incidence of peripheral nerve toxicity than the control group, the difference was statistically significant (χ2 = 13.93; P < 0.01). Chemotherapy effect between the 2 groups was no significant difference (P > 0.05). Conclusion: Treatment with air wave pressure therapeutic equipment can reduce the incidence of peripheral nerve toxicity during oxaliplatin chemotherapy.

Sarmentosin Induces Autophagy-dependent Apoptosis via Activation of Nrf2 in Hepatocellular Carcinoma

Background and Aims:Hepatocellular carcinoma(HCC)is a common and deadly cancer.Accumulating evidence supports modulation of autophagy as a novel approach for determining cancer cell fate.The aim of this study to evaluate the effectiveness of sarmentosin,a natural compound,on HCC in vitro and in vivo and elucidated the underlying mechanisms.Methods:Cell functions and signaling pathways were analyzed in HepG2 cells using western blotting,real-time PCR,siRNA,transmission electron microscopy and flow cytometry.BALB/c nude mice were injected with HepG2 cells to produce a xenograft tumour nude mouse model for in vivo assessments and their tumors,hearts,lungs and kidneys were isolated.Results:We found that autophagy was induced by sarmentosin in a concentration-and timedependent manner in human HCC HepG2 cells by western blot assays and scanning electron microscopy.Sarmentosin-induced autophagy was abolished by the autophagy inhibitors 3-methyladenine,chloroquine,and bafilomycin A1.Sarmentosin activated Nrf2 in HepG2 cells,as shown by increased nuclear translocation and upregulated expression of Nrf2 target genes.Phosphorylation of mTOR was also inhibited by sarmentosin.Sarmentosin stimulated caspasedependent apoptosis in HepG2 cells,which was impaired by silencing Nrf2 or chloroquine or knocking down ATG7.Finally,sarmentosin effectively repressed HCC growth in xenograft nude mice and activated autophagy and apoptosis in HCC tissues.Conclusions:This study showed sarmentosin stimulated autophagic and caspase-dependent apoptosis in HCC,which required activation of Nrf2 and inhibition of mTOR.Our research supports Nrf2 as a therapeutic target for HCC and sarmentosin as a promising candidate for HCC chemotherapy.