Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

Aeromonas hydrophila is a Gram-negative pathogen that can infect various fish,including largemouth bass(Micropterus salmoides),which have caused huge economic losses.In present study,largemouth bass anti-A.hydrophila antibodies were produced,then a highly immunogenic outer membrane proteins,AHA_3793,was identified by combined western blotting and mass spectrometry analysis.Moreover,AHA_3793 was expressed,and its immunogenicity was further verified by western blotting.Subsequently,the protective efficacy of AHA_3793 were evaluated in largemouth bass.The results showed that rAHA_3793 could produce a relative percentage survival(RPS)of 61.76% for largemouth bass against A.hydrophila challenge.ELISA analysis showed the specific serum antibodies of largemouth bass against rAHA_3793 and A.hydrophila in vaccinated group in weeks 4 and 5 after immunization were significantly higher than those in control group,which suggested that rAHA_3793 induced production of specific serum antibodies against rAHA_3793 and A.hydrophila.The qRT-PCR analysis showed that expressions of CD4-2 and MHC Ⅱα were also significantly up-regulated after immunization.These results collectively demonstrated that rAHA_3793 could induce a strong humoral immune response of largemouth bass,and then produce high immune protection ef fects against A.hydrophila infection.

通行能力退化路网中考虑ATIS信息影响的混合随机用户均衡模型研究（英文）

先进的出行者信息系统(简写ATIS)不仅能帮助出行者获取更准确的交通时间信息,还能提高出行者在面对路网通行能力下降等随机事件时的应急和实时调整能力。本文提出了一类新的混合随机用户均衡模型,用以描述在通行能力随机退化的路网中,装备ATIS和未装备ATIS两类出行者各自的路径选择行为和由此形成的路网交通流均衡状态。在提出的模型中,ATIS向出行者提供交通信息的优势体现在路网流量均衡分布状态的随机性更低,而ATIS对出行者适应路网能力随机退化的强化优势,则通过路网流量的多均衡态加以体现。论文将该混合随机用户均衡模型表示成一个关于混合路径流量的不动点问题,并设计了一类迭代求解算法。通过数值算例,论文分析了模型的相关数学特性,并验证了迭代算法的求解效率。