Status Quo and Countermeasures of Sustainable Development for Zhenjiang Wanshan Hongbian Modern Agricultural Science Park

In this paper,status quo of development in Zhenjiang Wanshan Hongbian Modern Agricultural Science Park is studied,and some problems are pointed out,such as unreasonable staffing structure and weak self-financing ability.Talent introduction strategy and branding and standardization strategy are proposed,and financing channels should be widened.Meanwhile,agricultural production structure should be adjusted,and science and technology services should be promoted.The research aims to provide the reference for sustainable development of similar modern agricultural science park in China.

Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola

Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice(Oryza sativa)production worldwide.Resistance to M.graminicola in rice could be the most effective option for its management.However,sources of germplasm with resistance to M.graminicola in rice remain limited.Here,we describe the root attraction,gall formation and genetic analysis of the resistance to M.graminicola in the rice variety Huidao 5.A nematode attraction assay showed that second-stage juveniles(J2s)of M.graminicola were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing 9108.Microscopic observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation,but their subsequent movement to the root tip was hindered in Huaidao 5,resulting in decreased nematode number compared to Nanjing 9108.Additionally,we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively,and found that gall number was significantly reduced,and nematode development was clearly suppressed in Huaidao 5.To investigate the genetic basis of this resistance,cross breeding was performed between the Huaidao 5 and Nanjing 9108 varieties.There was no reduction in the resistance of the F_(1) offspring to M.graminicola in the greenhouse or field trials,suggesting that a dominant gene could control resistance in Huaidao 5.In summary,this study provides a detailed characterization of a novel source of resistance to M.graminicola in rice,which is of great potential for use in crop breeding.

Seed Propagation Technique of Atractylodes lancea in Maoshan, Jiangsu

Due to over exploitation, the Atractylodes resources in such five counties and cities as Jurong, Jintan, Lishui, Liyang and Gaochun have been exhausted. This paper summarized the seed propagation technique of Atractylodes lancea in Maoshan, Jiangsu Province, aiming to provide reference for the restoration of A. lancea production in Maoshan, Jiangsu.

Research Progress on the Development of Modern Agricultural Science and Technology Park

This paper summarizes the typical models,construction theory and development status of modern agricultural science and technology parks at home and abroad,in order to provide a reference for the sustainable development of modern agricultural science and technology parks in China.It is found that at present,the agricultural science and technology park in China is in the stage of quantitative development,neglecting the qualitative construction of the park.This paper points out that the balanced relationship among ecology,economy and society should be adjusted to promote the sustainable development of the park.

Production of Rabbit Polyclonal Antibody Using hAPOA1 Protein Expressed in Prokaryotic Cells

The open reading frame （ORF） of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL21. The expression of target fusion protein was induced with isopropyl β-D-l-thiogalacto- pyranoside （IPTG）. The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay （ID-EL1SA）. ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1 ： 40 000 was produced, which may bring considerable economic benefits.

Microecological Prevention and Control Technology of Facility Strawberry Continuous Cropping Diseases and Its Application Effects

In order to actively develop green ecological strawberry, the authors have studied integrated microecological prevention and control technologies of strawberry continuous cropping diseases, including increasing soil biomass and solar high temperature disinfection during strawberry greenhouse leisure period in summer, biological fertilizer (agent) treatment before colonization, root irrigation treatment with biocontrol agent, as well as appropriate amount of fulvic acid and cytex after colonization, forming a supporting technical system for the microecological prevention and control of soil-borne diseases in facilities. The demonstration application in production shows that the technology has the functions of restoring and enhancing soil biological fertility, enriching beneficial microbial flora, antagonizing harmful bacteria, significantly promoting the growth and development of strawberry, reducing the incidence of soil-borne diseases, reducing the use of chemical fertilizers and pesticides, promoting the early ripening and high yield of strawberry, significantly improving the quality and flavor of strawberry, reducing the risk of pesticide residues, and boosting consumer confidence, which further improves the economic benefit, social benefit and ecological benefit, with good application prospect in production.

The Mitochondrial Genome of Raphanus sativus and Gene Evolution of Cruciferous Mitochondrial Types

To explore the mitochondrial genes of the Cruciferae family, the mitochondrial genome of Raphanus sativus （sat） was sequenced and annotated. The circular mitochondrial genome of sat is 239,723 bp and includes 33 protein-coding genes, three rRNA genes and 17 tRNA genes. The mitochondrial genome also contains a pair of large repeat sequences 5.9 kb in length, which may mediate genome reorga-nization into two sub-genomic circles, with predicted sizes of 124.8 kb and 115.0 kb, respectively. Furthermore, gene evolution of mitochondrial genomes within the Cruciferae family was analyzed using sat mitochondrial type （mitotype）, together with six other re-ported mitotypes. The cruciferous mitochondrial genomes have maintained almost the same set of functional genes. Compared with Cycas taitungensis （a representative gymnosperm）, the mitochondrial genomes of the Cruciferae have lost nine protein-coding genes and seven mitochondrial-like tRNA genes, but acquired six chloroplast-like tRNAs. Among the Cruciferae, to maintain the same set of genes that are necessary for mitochondrial function, the exons of the genes have changed at the lowest rates, as indicated by the numbers of single nucleotide polymorphisms. The open reading frames （ORFs） of unknown function in the cruciferous genomes are not conserved. Evolutionary events, such as mutations, genome reorganizations and sequence insertions or deletions （indets）, have resulted in the non- conserved ORFs in the cruciferous mitochondrial genomes, which is becoming significantly different among mitotypes. This work represents the first phylogenic explanation of the evolution of genes of known function in the Cruciferae family. It revealed significant variation in ORFs and the causes of such variation.