From field observations on eclogites towards modeling of deep subduction processes： 7th International Eclogite Conference （IEC-7） and First Workshop of Task Group Ⅱ-10 of lnternational Lithosphere Program

The 7th International Eclogite Conference was held in Seggau, near Leibnitz, Austria, from July 3-9, 2005, and was accompanied by inter-conference and post-conference field trips to the Tauren Window of the Alps. The Conference provided a comprehensive scientific forum in the spirit of previous conferences of this series with extended contributions related to highand ultrahigh-pressure metamorphism, and ultra-deep subduction of continental material to Earth＇s mantle. The first workshop of the new Task Group Ⅱ-10 （UDCCS--Ultra-Deep Continental Crust Subduction）, under the auspices of International Lithosphere Program, was held in conjunction with the conference. The Task Group Ⅱ-10 was established in 2005 to be continued to 2009. The conference was organized by Karl Franzens University of Graz, Austria, and supported by the following sponsors： Bundesministerium fur Bildungund Kultur, Fa. Bruker-AXS, Fa. Olympus, International Lithosphere Program, Land Steiermark Abteilung für Wissenschaft und Forchung, Osterreichische Mineralogische Gesellschaft, Raiffeisen Landesbank Steiermark and the University of Graz.

Suppression of intracranial glioma tumorigenesis with vascular endothelial growth factor antisense oligonucleotide in rats

Objective: To observe the inhibition of intracranial glioma tumorigenesis by vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (ODN) in rats. Methods: Totally 20 μ1 Hank's liquid containing 1×106 C6 glioma cells was seeded into rat right caudate putaraen in high-flow microinfusion with stereotactic technique. VEGF antisense ODN was simultaneously used with glioma cell. Each rat of the treated group Ⅰ and the treated group Ⅱ was treated with 1 000 μmol/L VEGF antisense ODN. Each rat of the treated group Ⅲ and the treated group Ⅳ was treated with 2 000 μmol/L VEGF antisense ODN. The experimental periods of the treated group Ⅰ , the treated group Ⅲ and the control group Ⅰ were 2 weeks, those of the treated group Ⅱ , the treated group Ⅳ and the control group Ⅱ were 3 weeks. Before sacrifice, MRI was performed on each rat. Tumor magnitude and pathologic examination were detected after samples were dissected. Results: The survival state of all treated rats was better, and that of the control rats was in severe danger. The tumor volumes of the treated group Ⅰ and the treated group Ⅱ were remarkably lessened. Tumor tissue could not be found macroscopically in the brain samples of the treated group Ⅲ and the treated group Ⅳ, but tumor nest could be found with microscopy. Tumors of the treated group I and the treated group Ⅱ had weak expressions of VEGF mRNA and VEGF, while normal brains and the samples of the treated group Ⅲ and the treated group Ⅳ had negative expressions, but tumors of the control groups had strong expressions. Conclusion: VEGF antisense ODN used early in situ can suppress angiogenesis and growth of rat intracranial glioma to retard tumorigenesis.

EXCIMER LASER IN SITU KERATOMILEUSIS FOR MYOPIA:ONE YEAR FOLLOW-UP

Objective To analyze the clinical effects after one year follow-up of LASIK for myopia. Methods According to the preoperative diopters of the myopia, patients, undergoing LASIK, were divided into three groups, group Ⅰ: 140 eyes ( -1. 25D ~ -6. 00 D) , group Ⅱ : 205 eyes ( -6. 25D ~ -10. 00D) , group Ⅲ: 226 eyes ( -10.25D- -15.00D). The patients were followed up at least I year. Results By groups, the percentage of the uncorrected visual acuity equal or more than 1. 0 were 94. 29% , 80. 30% , 42. 04% respectively. The residual refractive error within 0. 5D were 99. 29% , 85. 71% , 59. 70%. The spherical equivalent was stable 3 months after LASIK. The complications were few. Conclusion LASIK is a safe, effective and good predictable method for the treatment of myopia. It is one of the best choices to treat myopia below -10. 00D.

Effect of electrolyzed oxidizing water and hydrocolloid occlusive dressings on excised burn-wounds in rats

Objective: To study the efficacy of electrolyzed oxidizing water ( EOW ) and hydrocolloid occlusive dressings in the acceleration of epithelialization in excised burn-wounds in rats.Methods: Each of the anesthetized Sprague-Dawley rats (n = 28) was subjected to a third-degree burn that covered approximately 10% of the total body surface area. Rats were assigned into four groups: Group Ⅰ ( no irrigation), Group Ⅱ (irrigation with physiologic saline), Group Ⅲ ( irrigation with EOW ) and Group Ⅳ ( hydrocolloid occlusive dressing after EOW irrigation). Wounds were observed macroscopically until complete epithelialization was present, then the epithelialized wounds were examined microscopically. Results: Healing of the burn wounds was the fastest in Group Ⅳ treated with hydrocolloid occlusive dressing together with EOW. Although extensive regenerative epidermis was seen in each Group, the proliferations of lymphocytes and macrophages associated with dense collagen deposition were more extensive in Group Ⅱ, Ⅲ and IV than in Group Ⅰ. These findings were particularly evident in Group Ⅲ and Ⅳ.Conclusions: Wound Healing may be accelerated by applying a hydrocolloid occlusive dressing on burn surfaces after they are cleaned with EOW.

CsA down regulates IFN-γ gene transcription after liver transplantation by inhibiting NF-κB activity

Objective To investigate the relationship between NF-κB activity and IFN-γ gene expression, as well as the histopathological changes following liver transplants, both with and without cyclosporin A (CsA) treatment.Methods Sixty male Wistar and Thirty male SD rats were subjected to orthotopic liver transplants. Fourty-five of the Wistar rats were used as recipients, and were divided into 3 groups: group Ⅰ , syngeneic control (Wistar-to-Wistar); group Ⅱ , acute rejection (SD-to-Wistar); and group Ⅲ: acute rejection treated with cyclosporin A by intramuscular injection (SD-to-Wistar + CSA). After the liver transplants, electrophoretic gel mobility shift assay was used to analyze NF-κB activity in the splenocytes of recipient rats, and RT-PCR was used to measure IFN-γ gene expression in grafted liver specimens. In addition, histopathological examinations were performed to assess the severity of acute liver rejection.Results In group Ⅰ , low levels of NF-KB activity were only detectable on day 5 and 7 post-transplant, and only weak IFN-γ mRNA expression was observed at all time points. By contrast, both high NF-κB activity and high expression levels of IFN-γ mRNA were detected at all time points in group Ⅱ. In group Ⅲ, NF-κB activity and IFN-γ mRNA expression were significantly inhibited, as compared to group Ⅱ (P < 0. 05). A good correlation was found between NF-κB activity and IFN-γ mRNA expression (r = 0.815) . In addition, NF-κB activity and IFN-γ mRNA expression mirrored histopathological changes in all three experimental groups.Conclusions Changes in IFN-γ mRNA expression levels after liver transplantation are at least partially due to changes in levels of NF-κB activity. CsA appears to downregulate NF-κB activity, thus inhibiting IFN-γ gene transcription. Blocking the NF-κB mediated transcription pathway may be of benefit in preventing liver transplant rejection.