Anti-inflammatory effect of Diammonium Glycyrrhizinate in a rat model of ulcerative colitis

瞄准：为了探索 Diammonium 的反煽动性的机制，在 ulcerative 的一个老鼠模型的 Glycyrrhizinate 由乙酸导致了。方法：Spragur-Dawley 雌老鼠被划分成四个组：Diammonium Glycyrrhizinate 组，地塞米松组，乙酸控制和正常控制组。结肠的发炎被疾病活动索引，粗野词法损坏，组织学的损害和结肠的 myeloperoxidase 活动评估。Immunohistochemistry 被用来在结肠的粘膜检测 NF-kappaB， TNF-alpha 和 ICAM-1 的表示。结果：把控制，显示出的两 Diammonium Glycyrrhizinate 和地塞米松比作乙酸重要反煽动性的效果(P 【 0.01 ) 。在结肠的粘膜的 NF-kappaB， TNF-alpha 和 ICAM-1 的表示比在乙酸组在 Diammonium Glycyrrhizinate 组和地塞米松组是显著地更低的。结论：Diammonium Glycyrrhizinate 能在 ulcerative 的一个老鼠模型减少煽动性的损害。这可以在结肠的粘膜经由 NF-kappaB， TNF-alpha 和 ICAM-1 的抑制发生。

The Potential Effect of Puerarin in Preventing Atherosclerosis

Objective To study the activity of Puerarin (Pue) in scavenging oxygen free radical (OFR) and its inhibitory effect on the oxidative modification of low density lipoprotein (LDL). Methods Riboflavin-light system was used to generate superoxide anion, and Fenton reaction to generate hydroxyl free radical to study the activity of Pue in scavenging OFR. Hydrogen peroxide-induced hemolysis was used to study the effect of Pue on erythrocyte hemolysis and malondialdehyde (MDA) production. And ultraviolet ray and cupric sulfate were used to cause the oxidative modification of LDL for studying the inhibitory effect of Pue on LDL oxidative modification. Results (1) Pue could, at concentration of 0. 01 – 1.0 mmol/L, scavenge superoxide anion radical and at concentration of 7.5–75μmol/L scavenge hydroxyl radical in a concentration dependent manner. (2) Pue could, at concentration of 0.1–10 mmol/L, inhibit significantly oxidative hemolysis and MDA production of erythrocyte induced by hydrogen peroxide. (3) Pue of 0.01–1.0 mmol/L could inhibit the oxidative modification of LDL in a concentration dependent manner. Conclusion Pue has an anti-peroxidation effect and shows a potential effect in preventing atherosclerosis.