Classical garden as a kind of comprehensive art embodies a nation's culture and spirit, and culture has a direct impact on the style of classical gardens in the same period. Traditional Chinese and Korean gardens ...Classical garden as a kind of comprehensive art embodies a nation's culture and spirit, and culture has a direct impact on the style of classical gardens in the same period. Traditional Chinese and Korean gardens have intimate connection with each other and have their own unique systems. Also it is no exception in culture, that is, similar traditional cultures have different manifestations in their respective classical gardens. This study analyzes different manifestations of yin and yang and Five Elements Theory, thought of immortals and Confucianism in classical gardens of China and Korea.展开更多
Sustainability is an important objective for architecture as buildings contribute significantly to the quality of the environment.For this reason,various building environmental assessment systems have been developed a...Sustainability is an important objective for architecture as buildings contribute significantly to the quality of the environment.For this reason,various building environmental assessment systems have been developed and applied around the world since 1990.There is currently little research investigating newly developed systems unlike many previous studies on LEED,BREEAM,etc.To fill this gap,the present study focuses on the G-SEED system in Korea by comparing and contrasting its environmental categories and evaluation criteria with those of other major building environmental assessment systems.We introduce and examine the G-SEED system by addressing the Korean context and local needs to understand the forces between globalization and localization.Methods include a dense review of government documents and reports and interviews with practitioners.The results demonstrate that while the Korean rating system shares many similarities with other systems due to the influence of these predecessors on its development,the G-SEED has customized its practices to meet various local needs by developing unique evaluation criteria such as noise and safety.展开更多
An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2',4,4'-tetrabromodiphenyl ether ...An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in aqueous samples. A hapten, 2,4,2'- tribromodiphenyl ether-4'-aldehyde, was synthesized, and was conjugated to bovine serum albumin to form a coating antigen. Specific recognition of the antigen by anti-PBDE antiserum was confirmed by a surface plasmon resonance measurement. In the immunoassay, the coating antigen was adsorbed on a 96-well plate first, and a sample, antiserum and biotinylated goat anti-rabbit secondary antibody were then added and reacted sequentially. A biotinylated, double-stranded DNA with 219 base pairs was attached to the secondary antibody by using streptavidin as a molecular bridge. In situ multiple labeling of the antibody was accomplished after addition of a DNA-binding fluorescent dye, SYBR Green I. The working range of the immunoassay for the BDE-47 standard was 3.1-390 ~tg/L, with an IC50 value of 15.6 Ixg/L. The calculated LOD of the immunoassay is 0.73 Ixg/L. The immunoassay demonstrated relatively high selectivity for BDE-47, showing very low cross-reactivity (〈 3%) with BDE-15, BDE-153 and BDE-209. With a spiked river water sample containing 50 Izg/L BDE-47, quantification by the immunoassay was 41.9 ~tg/L, which compared well with the standard GC-ECD method (45.7 Ixg/L). The developed immunoassay provides a rapid screening tool for polybrominated diphenyl ethers in environmental samples.展开更多
基金Supported by Talent Initiation Project of Zhejiang A & F University Scientific and Research Development Fund:Preference Evaluation of Traditional Elements in Modern Landscape Spaces (2010FR078)
文摘Classical garden as a kind of comprehensive art embodies a nation's culture and spirit, and culture has a direct impact on the style of classical gardens in the same period. Traditional Chinese and Korean gardens have intimate connection with each other and have their own unique systems. Also it is no exception in culture, that is, similar traditional cultures have different manifestations in their respective classical gardens. This study analyzes different manifestations of yin and yang and Five Elements Theory, thought of immortals and Confucianism in classical gardens of China and Korea.
文摘Sustainability is an important objective for architecture as buildings contribute significantly to the quality of the environment.For this reason,various building environmental assessment systems have been developed and applied around the world since 1990.There is currently little research investigating newly developed systems unlike many previous studies on LEED,BREEAM,etc.To fill this gap,the present study focuses on the G-SEED system in Korea by comparing and contrasting its environmental categories and evaluation criteria with those of other major building environmental assessment systems.We introduce and examine the G-SEED system by addressing the Korean context and local needs to understand the forces between globalization and localization.Methods include a dense review of government documents and reports and interviews with practitioners.The results demonstrate that while the Korean rating system shares many similarities with other systems due to the influence of these predecessors on its development,the G-SEED has customized its practices to meet various local needs by developing unique evaluation criteria such as noise and safety.
基金supported by the Chinese Academy of Sciences (No. KSSCX2-YW-G-059)the National Hi-Tech Research and Development Program of China (No.2007AA06A407)the National Natural Science Foundation of China (No. 20825519, 20890112, 20921063)
文摘An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in aqueous samples. A hapten, 2,4,2'- tribromodiphenyl ether-4'-aldehyde, was synthesized, and was conjugated to bovine serum albumin to form a coating antigen. Specific recognition of the antigen by anti-PBDE antiserum was confirmed by a surface plasmon resonance measurement. In the immunoassay, the coating antigen was adsorbed on a 96-well plate first, and a sample, antiserum and biotinylated goat anti-rabbit secondary antibody were then added and reacted sequentially. A biotinylated, double-stranded DNA with 219 base pairs was attached to the secondary antibody by using streptavidin as a molecular bridge. In situ multiple labeling of the antibody was accomplished after addition of a DNA-binding fluorescent dye, SYBR Green I. The working range of the immunoassay for the BDE-47 standard was 3.1-390 ~tg/L, with an IC50 value of 15.6 Ixg/L. The calculated LOD of the immunoassay is 0.73 Ixg/L. The immunoassay demonstrated relatively high selectivity for BDE-47, showing very low cross-reactivity (〈 3%) with BDE-15, BDE-153 and BDE-209. With a spiked river water sample containing 50 Izg/L BDE-47, quantification by the immunoassay was 41.9 ~tg/L, which compared well with the standard GC-ECD method (45.7 Ixg/L). The developed immunoassay provides a rapid screening tool for polybrominated diphenyl ethers in environmental samples.