Simultaneous Determination of Six Components in Cortex Phellodendri by HPLC

[Objectives]This study was conducted to establish the method for simultaneous determination of six active components.[Methods]Simultaneous determination of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine in Cortex Phellodendri was carried out by HPLC with a Diamonsil C18(4.6 mm×250 mm,5μm)column was used.The mobile phase was acetonitrile-water(1‰acetic acid,2 mmol ammonium acetate)solution in gradient elution.The detection wavelength was set at 280 nm,and the column temperature was kept at 25℃and the flow rate was 1 ml/min.[Results]The linear ranges of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine were 20.00-320.00,18.75-130.00,25.00-200.00,5.00-100.00,20.00-200.00,and 0.09-1.80 mg/L,respectively.The average recovery was 98.1%,99.4%,97.5%,97.3%,104.0%,and 98.5%,respectively;and the RSDs were 0.5%,0.6%,0.8%,1.0%,1.4%,and 0.9%,respectively.[Conclusions]The method is convenient,stable,reliable and suitable for quality control of Cortex Phellodendri.

Effects of Different Application Rates of Bioorganic Fertilizer on the Growth of Spinach in a Short Term

[Objectives]This study was conducted to promote the rational use of bioorganic fertilizers.[Methods]Stanley bioorganic fertilizer was selected to investigate the laws and characteristics of the effects of bioorganic fertilizer on spinach growth with different application rates in a short term and found out the precise application rate.[Results]As the application rate of bioorganic fertilizer increasing,the emergence rate of spinach decreased.The total weights and leaf areas of spinach plants treated with different application rates of bioorganic fertilizers were all higher than those of the control check(CK).The total weights and leaf areas of spinach plants applied with bioorganic fertilizer at different rates were all higher than those of the CK.The total weight of spinach plants increased linearly with the application rate of bioorganic fertilizer increasing among treatments,while the leaf area fluctuated with the increase of bioorganic fertilizer concentration among various treatments.The average root volumes and average diameters of treatments applied with bioorganic fertilizer at different rates were all higher than those in the CK,but the average root length showed a different trend.The average root length was higher in the CK than in various bioorganic fertilizer treatments in the early growth period,but the differences of various treatments from the CK gradually decreased in the middle period,and in the later period,the root length of spinach treated with organic fertilizer gradually exceeded the CK.[Conclusions]This study provides a theoretical basis for the rational application of bioorganic fertilizers in spinach production.

X-ray Manifestations of Pneumoconiosis Patients and Differential Analysis with Pulmonary Tuberculosis

[Objectives]This study was conducted to explore the X-ray manifestations of pneumoconiosis patients and differentiation from pulmonary tuberculosis.[Methods]Retrospective analysis was conducted on 103 patients who underwent lung examinations,including 47 cases of pneumoconiosis and 56 cases of pulmonary tuberculosis.The differences in nodule characteristics and accompanying cavity sign between pneumoconiosis and pulmonary tuberculosis were compared.[Results]The X-ray manifestations of pneumoconiosis patients mainly included various forms of lung images,which were uneven in density,and had blurry edges,and sometimes thickening of lung markings and reduction of lung volume could be observed.The acinar nodules observed in X-ray films of patients with hematogenous disseminated pulmonary tuberculosis appeared petal like.The uniformity of nodule appearance such as nodule distribution,nodule density,and nodule size was significantly lower in pneumoconiosis than in hematogenous disseminated pulmonary tuberculosis,showing a statistical significance(P<0.05).The diameter of cavities in patients with pneumoconiosis accompanied by cavity sign[(0.64±0.13 vs 3.37±0.95)cm]was lower than that in patients with secondary pulmonary tuberculosis accompanied by cavity sign.There was a significant difference in the thickness of the cavity wall between the two diseases,and patients with pneumoconiosis accompanied by cavity sign had a higher rate of thick wall cavities(>3 mm).The X-ray manifestations of pneumoconiosis and tuberculosis were relatively similar,but the distribution,density and size of nodules in pneumoconiosis were relatively uneven,and the patients accompanied by cavity sign had smaller cavity diameter and higher rate of thick-walled cavities.[Conclusions]This study provides a reference for the clinical differential diagnosis of pneumoconiosis and pulmonary tuberculosis.

Nutritional Elements Related to Leaf Blight of Pearl Plum in Longtan Town

[Objectives]This study was conducted to investigate the correlation between leaf blight of pearl plum and nutrient elements from the perspective of plant nutrition.[Methods]Branch and leaf samples of pearl plum plants infected by or free of leaf blight and the corresponding 0-30 cm cultivated soil were collected,and statistical analysis was conducted according to the changes in nutrient contents and the incidence of leaf blight,so as to look for nutrients in pearl plum plants associated with leaf blight,as well as soil constraint factors.[Results]Phosphorus,iron and manganese in nutrients may be related to leaf blight,and the factors affecting leaf blight may be the ratios of P/Fe and P/Mn in leaves.[Conclusions]This study provides a basis for further research on the relationship between phosphorus,iron and manganese on the effect of leaf blight in pearl plum trees.

Expression of Hoxc13 Gene in Skin Tissue of Tan Sheep at Different Stages

[Objectives]The expression differences of Hoxc13 gene in the skin tissue of Tan sheep at different stages were analyzed. [Methods] qRT-PCR was used to analyze the mRNA expression level of Hoxc13 gene in the skin tissue of Tan sheep at different stages(35, 55 d), and the relative quantitative method was used to analyze the expression level. [Results] The content of Hoxc13 gene in the skin tissue of Tan sheep at 55 d was significantly higher than that in the skin tissue at 35 d. Hoxc13 gene was differentially expressed in the skin tissue of Tan sheep lambs at different stages(35, 55 d). [Conclusions] This study provides basic data for further exploration of the growth and development mechanism of Tan sheep.

Zinc Tolerance and Accumulation in Four Alfalfa(Medicago sativa)Varieties

To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The results showed that the Zn tolerance index of Aohan was significantly higher than other species.Among the four species,Aohan had the highest concentration of Zn in roots,followed by Golden Empress,Sanditi and Longxi.Aohan had the highest bioconcentration factor(BCF)in leaves.Whereas,Sanditi and Longxi had the lowest BCF in stem and roots,respectively.The translocation factor of Golden Empress was significantly lower than other species.The Zn accumulation rate of Aohan was higher than other species regardless of the concentration of Zn.Longxi had the lowest allocation of Zn in leaves and Golden Empress had the lowest allocation of Zn in roots.The concentrations of other elements(Fe and Mg)in leaves were decreased with Zn additions,but the interactions between Zn and other elements in roots varied with species.These results indicated that suitable species of alfalfa could successfully be used for the phytoremediation of Zn-contaminated soils.

Study on the Activation Effect of Fermented Organic Fertilizers on Selenium in the Soil of Southern Dragon Fruit Orchards

[Objectives]This study was conducted to explore the effects of using fermented organic fertilizers such as cow manure on the activity of selenium in the soil.[Methods]The project team applied the organic fertilizer prepared by fermenting cow manure and other raw materials to activate selenium in the soil at two dragon fruit orchard bases in Shilong Town,Guiping City and Dingdang Town,Long an County,and carried out many years of experiments.[Results]The contents of available selenium in the two experimental orchards in Shilong Town and Dingdang Town increased by 10%and 5%,respectively,and the selenium contents in dragon fruit increased by 228.6%and 200.0%,respectively.[Conclusions]The application of fermented organic fertilizers such as cow manure can increase available selenium content in the soil,thereby increasing the selenium content of dragon fruit.

Study on Variation Characteristics and Prediction Model of Stem Sap Flow of Jujube Under Different Injection Irrigation Models

[Objectives]This study was conducted to study the variation laws and characteristics of stem sap flow rate in jujube under the injection irrigation mode,to improve the growth quality of jujube trees.[Methods]According to the characteristics of the growth environment and physiological characteristics of jujube Junzao trees in southern Xinjiang,eight different injection irrigation modes were designed.Through the variation of stem sap flow rate,the most suitable irrigation method for jujube Junzao trees in southern Xinjiang was explored.The variation law of stem sap flow rate of jujube and the effect of meteorological factors on stem sap flow rate of jujube were studied.The variation law of stem sap flow in jujube trees was analyzed,and multiple regression models and time series models were established to predict stem sap flow of jujube trees.[Results]The results showed that the simulation results of the time series model were closer to the actual data,and the model could accurately predict the stem sap flow rate of Junzao.[Conclusions]This study is suitable for the full utilization of water resources in the Tarim Basin in southern Xinjiang,and has important practical significance for analyzing the effect of water stress on the growth and development of jujube trees and precision irrigation.

Synthesis Design and Research Progress of MOF Materials in Biomedicine

Compared with traditional materials,metal-organic framework materials(MOFs)have the characteristics of drug release controllability,degradability,designability and adjustability of composition and structure,excellent load capacity,and designability and adjustability of channel shape and size,and have shown a wide range of application value in the field of biomedicine.In this paper,based on the structural characteristics of MOFs,the synthetic design of MOF materials was expounded,and the research achievements of MOF materials in biomedicine in recent years were reviewed.

Research Progress and Prospect on the Effects of Abiotic Stress on Plant Photosynthesis

Photosynthesis is the basis of plant growth and development as well as the existence of the biological world. Photosynthesis is of great theoretical and practical significance. In this paper, the effects of temperature, drought, salt, light and other abiotic stress factors on plant photosynthesis were reviewed.

Development of Whole Potato Flour Fish Noodles

[Objectives] This study was conducted to prepare a kind of traditional aquatic food―fish noodles in Hubei Province by replacing some wheat flour with whole potato flour. [Methods] With whole potato flour, wheat flour and minced fish as main raw materials, the effects of the ratio of whole potato flour to minced fish and the addition amounts of sodium alginate, egg white, salt and ginger juice on the quality of whole potato flour fish noodles were studied. An orthogonal experiment was conducted to confirm the optimal technical formula of the whole potato flour fish noodles. [Results] The best formula of the whole potato flour fish noodles was whole potato flour 30 g, wheat flour 20 g, minced fish(grass carp) 50 g, salt 2.00 g, egg white 4.00 g, sodium alginate 2.00 g, and ginger juice 5.00 g. [Conclusions] The whole potato flour fish noodles prepared according to this formula are not only nutritious and taste good, but also have the advantages of easy and convenient eating and storage. This kind of fish noodle has good development prospects.

Evaluation of Ecological Environment Quality of Typical Forest Parks

Forest tourist areas have an excellent ecological environment,which is conducive to people's physical and mental health.In this study,Fuzhou National Forest Park and Wuying National Forest Park were selected as typical representatives of the southern and northern forest parks,respectively,which were compared by qualitative and quantitative research methods from such five aspects as air quality,climate quality,water environment quality,acoustic environment quality and biological population in some forest areas.The results showed that Fuzhou National Forest Park has a concentration of negative oxygen ions much higher than Wuying National Forest Park,and is thus more conducive to people's health;and it has the characteristics of warm and comfortable climate throughout the year and high biodiversity,but the noise is 4.35 db higher than Wuying National Forest Park,and the water quality is slightly poorer than that of Wuying National Forest Park.And its DO value is about 3.89 smaller in autumn than in summer.In Wuying National Forest Park,the concentration of negative oxygen ions is 53.6% lower than Fuzhou National Forest Park,but it is also above the national standard.The climate is only comfortable in June-August,and is colder or cooler in other months.The water quality is better than that of Fuzhou National Forest Park.In particular,the PH value is about 0.38 lower than that of Fuzhou National Forest Park.It has its own unique biological characteristics.The number of tourists in summer is higher,resulting in higher noise in summer than in autumn.The two forest parks in the north and the south are both good places for people to relax and enjoy.

Conditional Optimization of Laccase Production by Whiterot Fungi through Fermentation

Phanerochaete chrysosporium was selected as the production strain of laccase,and the effects of stirring speed,ventilation volume,culture temperature,inoculation amount and initial p H of medium on laccase production by liquid fermentation in cylinder were studied.On the basis of single factor test,an orthogonal test was carried out to find optimal conditions for laccase production P.chrysosporium through liquid fermentation.These results showed that the stirring speed of fermentation cylinder had the highest effect on laccase production,and the optimal conditions were shown as follows:the temperature at 28℃,the rotating speed at 300 r/min,the ventilation volume of 5 L/min(ventilation ratio of 1.0 vvm),the initial p H of medium of 5,and the inoculation amount of 15%,which gave the highest laccase level of 14.86 U/ml.

Effects of Colchicine on Callus Induction and Differentiation of Erianthus arundinaceus

Erianthus arundinaceum, a related species of the most important sugar crop Saccharum, and is an essential gene resource for stress and disease resistance in sugarcane breeding. Colchicine is the most common and effective chemical inducer in polyploid breeding at present. To detect the effects of colchicine on callus induction and shoot regeneration, E. arundinaceus ＇ Yun 83-183＇, with the chromosome of 2n = 60, was used as the material for studying the effect of different concentrations of 2,4-D and colchicine, treatment time and culture methods on callus induction and shoot regeneration. The results indicated, when the callus was cultured for 7 d in solid medium with 0.07% colchicine or 0.5 d in liquid medium with 0.05% colchicine, callus had no ability of shoot regeneration, which meant that in liquid culture, colchicine had not only high inhibitory effect on callus, but also lower concentration and shorter treatment time than solid culture; when the concentration of colchicine was 0.03% and callus was cultured for 7 d in solid medium or 0.5 d in liquid mhdium, the resistant callus rate and resistant bud rate were the highest, although the resistant callus rates were all about 88.00% and had no significant difference between solid culture and liquid culture, the resistance bud rate was only 6.00% in liquid culture, 16.22% lower than in solid culture, which meant that solid culture was more effective than liquid culture at the same colchicine concentration. For E. arundinaceus ＇ Yun 83-183＇ , the best concentration of 2,4-D was 2 mg/L, the best concentration of colchicine was 0.03%, and the best effect of callus induction and shoot regeneration was achieved within 7 d of solid culture. This study would provide fundamental information for chromosome doubling of E. arundinaceus in the future.

Discussion on Individual Expected Maturity of Populus canadensis Moench

Through the study of parse wood materials in Shandong Province, the fitting empirical equation of tree growth was obtained, a function with tree growth was a variable and time as an independent variable. Through mathematical operations such as function derivation, the mature age of tree growth was obtained. The obtained expected maturity age for Populus canadensis Moench forest was 11 a for pulp material, 26 a for pillar materials and 41 a for peeler. And the application, research directions and precautions of the mature ages were proposed.

Nutrient Composition of Different Foxtail Millet Seeds

Ten millet cultivars from China and abroad were analyzed for their crude fat, amylose and amylopectin, crude protein and amino acid content. A wide variation was observed in amylopectin contain, ranging from 54.47% to 69.26%, while amylose content ranged from 11.26% to 23.22%. The average crude fat in all cultivates was 3.46%, and most cultivates ranged from 3.1% to 3.7%. Protein contents ranged from 8.61% to 15.54% with a mean value of 11.94%. For amino acid composition, the ten cultivates were particularly rich in leucine, and the mean was 34.57 mg/g. The millet cultivars were also high in glycine, glutamic acid and cysteine. Millet was known to be limiting in the essential amino lysine, and the mean was 5.18 mg/g. Other amino acids, tyrosine, histidine and arginine were also very low in the ten cultivars. In general, significant nutrient composition differences were observed between the different millet cultivars, and A2 （a kind of mother line of the hybrid millet） and hybrid millet registered a higher level of protein and amino acid composition which would be useful in millet breeding.

Study on Correlation between Increment of Larix gmelinii and Annual Precipitation

Through the study on data of analytic tree, the fitting empirical equation for tree growth was obtained, i.e. , the function with increment as variable and annual precipitation as the independent variable. The threshold value of annual precipitation for tree growth was obtained through mathematical operation including derivation. It was concluded that Larix gmelinii grows the fast under the annual precipitation of about 545.1 mm, and negative growth would occur if the annual precipitation is lower than 349 mm and higher than 1 132.8 mm. Furthermore, the application value, research direction and matters needing attention were pointed out.

Preliminary Study on the Control of Kiwifruit Canker by 6%Ascorbic Acid Water Aqua

[Objectives]This study was conducted to investigate the control efficacy of 6% ascorbic acid water aqua on kiwifruit bacterial canker. [Methods]The ascorbic acid water aqua was sprayed onto annual potted kiwifruit seedlings once every 5 d,for three times in total,and Pseudomonas syringae pv. Actinidiae was inoculated by needle punching 7 d later. Scab diameter and number of newly infected scab were investigated to analyze the control efficacy. [Results] Kiwifruit plants were treated with 6% ascorbic acid water aqua,and after 30 d,the 20 mg/L treatment showed an incidence rate of 25. 5%,and the 60 mg/L treatment exhibited an incidence rate only of 12. 1%; and after 60 d,the relative control efficacy reached 90. 7% at the mass concentration of 60 mg/L. [Conclusions] The6% ascorbic acid water aqua weakened the destruction and expansion of the pathogen in plants,effectively improved the resistance in kiwifruit plants,and had certain control efficacy on kiwifruit canker.

Effects of Two Silicon Fertilizers on Maize Growth and Tetranychus truncatus Ehara

[ Objectives] This study was conducted to investigate the effect of silicon fertilizer on maize growth and Tetranychus truncatus Ehara. [ Methods ] Yougui and Guishen were selected and sprayed onto maize for 1, 2 and 3 times, forming difference treatments. [ Results ] Spraying the two kinds of silicon fertilizers for different times promoted the growth of plant height, leaf length, leaf width and leaf thickness of maize, increased chlorophyll contents in maize leaves and yield of single ear, and also adversely affected T. truncatus （i. e. , increased the death rate of T. truncatus）. The more the treatment times of silicon fertilizer, the more remarkable the effect, and the higher the adverse effect on T. truncatus. [ Conclusions] In production, spraying silicon fertilizer onto maize for 2 -3 times could realize the purposes of increasing maize yield and alleviating the danlage by T. truncatus.

Histological Observation of Chicken Skin

In this study, histological characteristics of the skin of adult lsa laying hens were observed. The results show that chicken skin is composed of epider- mis, dermis and hypodermis. The epidermis of chicken skin is thin, consisting of basal cells, spiny cells, transitional cells and horny cells. Specifically, there are few spiny cells scattered between the stratum basale and the transitional layer, without forming a layer; only 1 -2 layers of cells can be observed in the transitional layer. The derails is divided into superficial dermis and deep derufis. Moreover, deep dermis consists of dermal dense layer, dermal loose layer and elastic fibrous layer. There is no smooth muscle in pteryla within the dermal dense layer. The loose layer of deep dermis is developed, which is much much thicker than the dense layer. This study laid the foundation for in-depth investigation of the histological structure of poultry skin.