Treatment of permanent pacemaker electrode-induced cardiac perforation

A case of permanent pacemaker electrode-induced perforation in the Third Affiliated Hospital of Inner Mongolia Medical University was collected and analyzed on the basis of diagnosis,physical examination and treatment.Cardiac perforation is one of the most serious complications of pacemaker implantation.As this disease is rare,it is easy to be misdiagnosed or missed diagnosis.We hope to improve the clinician’s awareness and vigilance of permanent pacemaker electrode perforation by analyzing the data of this case.

Study on the mechanism of microporous sheep ADM combined with hUCMSC in promoting wound healing

Objective:To explore the therapeutic mechanism of microporous sheep acellular dermal matrix(ADM)combined with human umbilical cord mesenchymal stem cell(hUCMSC)to promote the healing of full-thickness skin defect.Methods:hUCMSC was co-cultured on microporous sheep ADM to form composite biological dressings.Seventy-two nude mice were selected to make full-thickness skin injury models and randomly divided into 3 groups(hUCMSC+microporous sheep ADM group,sheep ADM group and iodophor gauze group),with 24 mice in each group.The wound healing rate of each group was detected at 14 d,21 d and 28 d after operation,qRT-PCR technique was used to detect the expression of Bax and Bcl-2 in the wound tissues,and the immunohistochemical staining technique was used to detect the expression of Collagen I and vascular endothelial growth factor(VEGF).Data were analyzed with one-way ANOVA and t test.Results:At 14 d after operation,the wound healing rate of the hUCMSC+microporous sheep ADM group was(65.34±14.72)%,which was significantly higher than that of the iodophor gauze group[(37.54±10.21)%],and higher than that of the sheep ADM group[(49.08±11.16)%],the differences were statistically significant(t=19.52,14.72;p<.05).With the gradual healing of the wound,at 28 d after operation,the wound healing rate of the hUCMSC+microporous sheep ADM group was(98.63±15.41)%,which was significantly higher than that of the iodophor gauze group[(81.74±16.27)%],and higher than that of the sheep ADM group[(63.47±14.80)%],the differences were statistically significant(t=-16.42,20.35;p<.05).The expression of Bax in the wound tissues of the hUCMSC+microporous sheep ADM group was significantly reduced,especially at 21 d after operation,the expression level was 0.25±0.06,which was significantly lower than the iodophor gauze group(0.53±0.16)and the sheep ADM group(0.41±0.12),the differences were statistically significant(t=3.52,-2.83;p<.05).The expression of Bcl-2 in the wound tissues of the hUCMSC+microporous sheep ADM group was significantly higher than those of the other two groups,especially at 21 d after operation,the expression level was 0.63±0.19,which was significantly higher than the iodophor gauze group(0.34±0.09)and the sheep ADM group(0.46±0.13),the differences were statistically significant(t=5.31,-6.07;p<.05).Immunohistochemical detection showed that the expression of Collagen I and VEGF in the hUCMSC+microporous sheep ADM group was slightly more than that of the sheep ADM group and the iodophor gauze group,but the effect was not remarkable.Conclusions:hUCMSC+microporous sheep ADM composite dressing can promote the healing of the full-thickness skin injury and reduce the production of apoptotic cells by carrying hUCMSC.

Initial research of cord blood leptin, adiponectin and IGF-I with fetus growth and development

Objective:In order to reveal the relationship between cord blood leptin,adiponectin and insulin-like growth factor-I(IGF-I)and the fetus growth and development,and discuss the interaction and clinical significance on fetus growth and development.Methods:The levels of cord blood leptin,adiponectin,IGF-I in 86 newborns were examined by radio immunoassay,according to gestation age and birth weight percentile relation,the objects were divided into the SGA group(n=16),the AGA group(n=41),the LGA group(n=29),meanwhile,neonatal birth weight,body length,head circumference,foot length,and placental weight were measured,and body mass index(BMI)was computed.Dependability analysis was taken.Results:The levels of cord blood leptin,adiponectin and IGF-I were as follows:LGA group>AGA group>SGA group.The level of cord blood adiponectin was positively correlated with birth weight,placental weight and BMI(p<.05).Cord blood leptin and IGF-I concentrations were positively correlated with their birth weight,body length,head circumference,foot length,placental weight and BMI,respectively(p<.01),cord blood leptin was positively correlated with adiponectin and IGF-I(p<.01).The levels of cord blood leptin and adiponectin had no statistical significance with neonatal sexuality and deliver style(p>.05);the levels of cord blood IGF-I had no statistical significance with neonatal sexuality(p>.05),but had statistical significance with deliver style(p<.05).Conclusions:Cord blood leptin,adiponectin and IGF-I played an important part in adjusting fetus growth and development as well as participating in the process of fetus growth and development,and could be regarded as one of the clinical indexes to evaluate fetus growth and development or state of nutrition.The abnormal level of cord blood leptin and IGF-I might be one of the reasons to cause intrauterine growth retardation and fetal macrosomia.

The study of myocardial protective effect of creatine phosphate sodium in liver transplant patients

Objective:To study the cardioprotective effectof creatine phosphate sodium by measuring perioperative myocardial enzymes of allogeneic liver transplant patients.Methods:The 26 cases of orthotopic liver transplant patients were randomly divided into control group and creatine phosphate sodium group,13 cases in each group.The creatine phosphate sodium group were given creatine phosphate sodium 30 mg/kg at the time of induction.The control group were given equal amount of N.S.All patients at six-phase of before surgery,after anesthesia,before anhepatic phase,anhepatic phase,new liver and the 12 hours after surgery extraction radial artery.The CK,CK-MB and cTnI activity was measured.Results:To compared with control group,creatine phosphate sodium group’s plasma CK,CK-MB and cTnI concentrations in before anhepatic phase,anhepatic phase,new liver and the 12 hours after surgery 4 phase are significantly lower(p<0.05).Conclusions:The creatine phosphate sodium has a protective effect on liver transplantation in patients with myocardial damage.

Expression of toll-like receptor 4 gene polymorphism and inflammatory factors in peripheral blood on patients with sepsis

Objective:To observe the presence of toll-like receptor 4(TLR4)gene polymorphism in the venous blood in patients with sepsis,meanwhile,to observe the expression and the diagnostic value of TLR4 mRNA,interferon-(IFN-),interleukin-23(IL-23),procalcitonin(PCT)and C-reactive protein(CRP)in patients with different degrees of sepsis.Methods:The peripheral blood samples from the subjects were collected to extract genomic DNA,gene sequencing and enzyme digestion method were applied to the detection of TLR4 Asp299Gly loci polymorphism.The expression of TLR4 mRNA in the peripheral blood was detected by reverse transcriptase polymerase chain reaction(RT-PCR).The expression levels of IFN-,IL-23,PCT and CRP were measured by enzyme linked immunosorbent assay(ELISA).Results:(1)The gene polymorphism was not present in TLR4 Asp299Gly loci;(2)The expression of TLR4 mRNA in the peripheral blood in patients with sepsis:on d1,there were statistically significant differences between the normal group and the group(APACHE II≤20),between the normal group and the group(APACHE II>20),between the group(APACHE II≤20)and the group(AAPACHE II>20)(t=5.741,14.780 and 10.500,all p<.01).APACHE II stands for Acute Physiology and Chronic Health Evaluation II.On d7,there were statistically significant differences between the normal group and the group(APACHE II≤20),between the normal group and the group(APACHE II>20),between the group(APACHE II≤20)and the group(APACHE II>20)(t=4.186,13.830 and 9.645,all p<.01);(3)The expression levels of IFN-,IL-23,PCT and CRP were obviously upregulated(all p<.01),and TLR4 was positively correlated with IFN-and IL-23(all p<.01);(4)The best cutoff value of TLR4 mRNA at baseline was 891.6μg/L,with a sensitivity of 100%and a diagnostic specificity of 57%.The best cutoff value of IFN-at baseline was 84.5μg/L,with a sensitivity of 100%and a diagnostic specificity of 57%.The best cutoff value of IL-23 at baseline was 861μg/L,with a sensitivity of 100%and a diagnostic specificity of 97%.Conclusions:(1)The Asp299Gly polymorphism is not present in TLR4 gene;(2)The expression levels of TLR4 mRNA,IFN-and IL-23 are relatively high in patients with sepsis,and will be higher with the increased severity of sepsis;(3)TLR4 mRNA,IFN-and IL-23 can be used as molecular biomarkers for the early diagnosis of sepsis.

A study on the relationship between changes in serum hs-CRP levels and Chinese ischemic stroke subclassification

Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were isolated from umbilical cord tissues of full-term healthy fetuses after cesarean section and then cultured. Cells in the third passage were chosen for the use of experiment after identification. SA strains isolated from wounds of burn patients in our burn wards were used in the following experiment. Cells were divided into 0, 10, 100 and 1,000 ng/ml lipopolysaccharide (LPS) groups by use of the random number table (similarly hereinafter). Cells were cultured with culture medium containing mesenchymal stem cells (MSCs) after being treated with medium containing corresponding mass concen-trations of LPS for 12 h. At post culture hour (PCH) 6, 12 and 24, 6 wells of culture supernatant of cells in each group were obtained to measure the content of LL-37 with enzyme-linked immunosorbent assay (ELISA). Ninety blood agar culture plates were divided into ciprofloxacin control group (CC), ciprofloxacin + supernatant group (CS), and ciprofloxacin + supernatant+ LL-37 antibody group (CSL), with 30 blood agar culture plates in each group. Blood agar culture plates in group CC were coated with 1.5 × 108 colony forming unit (CFU)/ml bacteria solution prepared with normal saline. Blood agar culture plates in group CS were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline and hUCMSC culture supernatant (cultured by MSC culture medium, the same below) in double volume of normal saline. Blood agar culture plates in group CSL were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline, hUCMSC culture supernatant in double volume of normal saline, and 2.6 μL of LL-37 antibodies at the concentration of 2 μg/ml. At PCH 12, 24 and 48, 10 blood agar culture plates were taken out from each group to observe the distribution of SA colonies on blood agar culture plates and to measure diameters of zones of inhibition of ciprofloxacin. The minimum inhibitory concentration (MIC) of ciprofloxacin against SA in each group was recorded. Fractional inhibitory concentration (FIC) indexes of ciprofloxacin in group CS and group CSL at PCH 12, 24 and 48 were calculated, with the synergistic effect evaluated. Data were processed with factorial design ANOVA, one way ANOVA, LSD-t test, Kruskal-Wallis test and Mann-Whitney U test. Results: (1) At each PCH, the content of LL-37 in cell culture supernatant in 10 ng/ml LPS group, 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 0 ng/ml LPS group (with t values ranging from 11.22 to 33.36, p values all below .01);the content of LL-37 in cell culture supernatant in either 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 10 ng/ml LPS group (with t values ranging from 2.24 to 18.73, p < .05 or p < .01);the content of LL-37 in cell culture supernatant in 1,000 ng/ml LPS group was higher than that in 100 ng/ml LPS group (with t values ranging from 12.46 to 14.70, p values all below .01). (2) At PCH 12, 24 and 48, the bacterial colonies in groups CC, CS and CSL became integrated over time. In CC group, diameters of zones of inhibition of ciprofloxacin at PCH 12, 24 and 48 were 26 mm, 24 mm and 23 mm respectively, with no obvious changes. At PCH 12, 24 and 48, diameters of zones of inhibition of ciprofloxacin in groups CS and CSL were 82 mm, 71 mm, 68 mm and 74 mm, 59 mm, 56 mm respectively, which were significantly larger than those in group CC. (3) At each PCH, MIC of ciprofloxacin against SA in group CC was significantly higher than that in groups CS and CSL respectively (with Z values ranging from 6.22 to 6.71, p values all below .01);MIC of ciprofloxacin against SA in group CSL was significantly higher than that in group CS (with Z values all equal to 6.72, p values all below .01). (4) FIC indexes of ciprofloxacin in groups CS and CSL at PCH 12, 24 and 48 were 0.011, 0.032, 0.032 and 0.122, 0.350, 0.350, respectively. The results indicated that hUCMSC culture supernatant had a synergistically antibacterial effect when combined with ciprofloxacin. Conclusions: hUCMSCs can secrete LL-37, and the secretion level is improved with increase of LPS concentration. The com-bination of hUCMSC culture supernatant with ciprofloxacin can decrease the dosage of ciprofloxacin in resisting SA effectively. Once LL-37 is neutralized, the synergistically antibacterial effect of hUCMSC culture supernatant is decreased thereby.

Effects of psychological intervention on glucose metabolism in patients with type 2 diabetes mel?itus accompanying depression in community

Objective: To explore the effect of psychological intervention on glucose metabolism in patients with type 2 diabetes mellitus (T2DM) accompanying depression in community. Methods: Four hundred and ten cases of T2DM patients in the Community Health Service Center of Friendship Office (Bao-tou) were chosen as the research objects. The evaluation and analysis were made according to Zung self-rating depression scale (SDS), and 176 cases of T2DM patients with depression were found out in the community. 176 cases of T2DM patients with depression were divided into the experimental group and the control group by use of random number table, with 88 cases in each group. The control group was treated with conventional therapy, while the experimental group was given psychological intervention treatment on the basis of conventional therapy. After 3-month psychological intervention, fasting blood glucose (FPG), two-hour postprandial blood glucose (PG2h), glycosylated hemoglobin (HbA1c) and Zung self-rating Depression Scale (SDS) were measured in two groups. In two months after psychological intervention stopped, the follow-up was conducted to determine the levels of FPG, PG2h and HbA1c, and to assess the level of depression and quality of life. Statistical analysis was performed by use of SPSS 17.0 statistical software. Results: After 3-month psychological intervention, the levels of FPG, PG2h and HbA1c in the experimental group were sig-nificantly lower than those in the control group, the depression index was significantly lower than that in the control group as well, the difference was statistically significant (p < .01). In two months after psychological intervention stopped, glucose metabolism indicators in the experimental group were significantly lower than those in the control group;the depression index was dramatically lower than that in the control group, the difference was of statistical significance (p < .01). Conclusions: Psychological intervention can effectively improve the depression symptoms and glucose metabolism indicators in T2DM patients with depression in community, but it needs a long-term persistence.

Dendritoma therapy of transferrable brain glioma in mice: An experimental study

Glioma is the most common central nervous system primary tumor,accounting for about 40%of intracranial tumors.In recent years,studies have shown that the human brain is not immune-free zone.The low immunogenicity of the tumor itself,tumor-generated immunosuppressive factors,and the low immunity of tumor patients all contribute to the“immune escape”phenomenon of glioma cells.Dendritic cells(DCs),as the strongest antigen-presenting cells in the body,play an important role in tumor immunotherapy with T-cell recognizing tumor antigens as the core.Therefore,it is necessary to adopt a strategy of culturing and sensitizing DC cells in vitro to increase their number and improve their function before returning to the body for treatment of patients with glioma.

Clinical study on treatment of TOSSY type III acromioclavicular joint dislocation with clavicular hook plate combined with coracoclavicular ligament repair

Objective:To explore the treatment of TOSSY type III acromioclavicular joint dislocation with clavicular hook plate combined with coracoclavicular ligament repair and its clinical effect.Methods:80 patients with TOSSY type III acromioclavicular joint dislocation who were admitted to our hospital from January 2014 to January 2019 were selected.They were randomly divided into the control group(n=39,treated with clavicular hook plate)and the observation group(n=41,treated with clavicular hook plate combined with coracoclavicular ligament repair)by the random number table method.The clinical efficacy in 12 months after operation,related clinical indicators,12-month postoperative recovery and postoperative complications were compared between the two groups.Results:The excellent and good rate was 92.68%(38/41)in 12 months after operation in the observation group,which was higher than that(76.92%,30/39)in the control group(p<.05).There were no significant differences in the duration of surgery,intraoperative blood loss and HLOS between the observation group and the control group(p>.05).In 12 months after operation,abduction activity and forward flexion activity of the observation group were higher than those of the control group,and visual analogue score(VAS)was lower than that of the control group(p<.05).The incidence of complications in the observation group was slightly lower than that in the control group,but there was no statistically significant difference between the two groups(p>.05).Conclusions:For TOSSY type III acromioclavicular joint dislocation,clavicular hook plate combined with coracoclavicular ligament repair has a good clinical efficacy.It can improve shoulder joint function,alleviate shoulder pain and have fewer complications.It can provide a reference for clinical treatment of this type of acromioclavicular joint dislocation.

Case report–Prolonged SARS-CoV-2 positive PCR after resolution of symptoms

The recent SARS-CoV-2 pandemic is a new challenge for clinicians worldwide.Although the clinical aspects are well described by different groups,there are still some areas of uncertainty about the interpretation of diagnostic methods.Herein,we describe the clinical case of a 45-year-old lady that contracted SARS-CoV-2 infection with a prolonged PCR positive nasopharyngeal swab but lack of COVID-19 defining events.

One-piece implant-supported overdenture in fully edentulous patients: Report of two cases

Dental implants with a reduced diameter can be placed in regions that lack adequate bone volume enabling functional and aesthetic oral rehabilitation without the complicated bone augmentation procedures.This article describes surgical and prosthetic procedures used in oral rehabilitation using Straumann R one-piece Mini Implants(2.4-mm diameter with Optiloc R prosthetic connection system)in two fully edentulous women aged 57 and 74.Both patients presented with severe ridge resorption.Ten one-piece mini dental implants(six on maxillary arch and four on mandibular arch)were placed in each patient to support new complete overdentures.Both patients were delighted with the results and regained their social lives.In addition,stabilized dentures allowed the patients to regain fundamental functions of the mouth:chewing and verbal(speech)and non-verbal(smiling)communication without the fear or difficulty experienced while wearing dentures that can move.In conclusion,mini implants offer an efficient,economical,and less invasive solution,especially for edentulous patients with atrophic bone structure or who cannot undergo complex surgical procedures.

Experimentation of acute injured spinal cord by transplanting the bone marrow stromal cells

Objective:In this study,bone marrow stromal cells(BMSCs)were transplanted in situ after spinal cord injury(SCI)in rats.It was proved that the implanted BMSCs could differentiate into neuron-like cells in the injured spinal cord,and the long-term motor ability test was carried out to investigate the recovery of neurological dysfunction after transplantation.Methods:(1)An improved Alien’s SCI rat model(weight 10 g,height 30 mm)was made by Alien weight drop method.Methyl eosin(Haematoxyli-n/eosin,HE)was used to identify with SCI.(2)The BMSCs were identified by the method of bone marrow adherent culture in vitro.The morphology of the cells was observed by cell staining,and CD44 was detected by BMSCs.After three cultures,the cells were transfected with lentiviral vectors carrying Green Fluorescent Protein(GFP)gene.(3)Cell survival after passage and transplantation:the GFP labeled BMSCs in situ(injury zone)were implanted into the injury model.The spinal cord sections were sacrificed after 2 weeks(2w),4 weeks(4w)and 6 weeks(6w)respectively,and the cells expressing GFP were observed under immunofluorescence microscope.(4)The modified Rivlin oblique plate test and spinal motor function blood brain barrir(BBB)score method were used to compare the changes of exercise ability in the simple injury group,2w after transplantation group,4w after transplantation group and 6w after transplantation group.Results:(1)The Alien’s SCI rat model was made by using Alien’s heavy drop method,and the effect was reliable and stable.(2)The proliferation of BMSCs after 3 passages has stabilized.After culture and amplification in vitro,the cell morphology changed to be conical,protruding interwoven into a network.Therefore,it could be used as a source of cell transplantation after SCI.The morphology of the cells was observed by staining,and the positive CD44 was detected.(3)BMSCs were transfected into the SCI model by lentiviral vector carrying GFP gene,and the expression of GFP in 6w was observed.(4)The results of modified inclined plane test and Rivlin locomotor BBB score of rats in simple injury group were significantly worse than those in transplantation control group(p<.05).2w,4w and 6w after transplantation groups could improve long-term motor function.The recovery of neurological dysfunction after 6w was the best,and the difference was statistically significant(p<.05).Conclusions:BMSCs transplantation could promote the recovery of neurological dysfunction after SCI,and the mechanism may be related to the differentiation of BMSCs into neurons and glial cells,and the BMSCs induced by transplantation could interact with peripheral nerve cells and produce some cytokines.

Comparative study of conventional urosonography without contrast enhancement and x-ray voiding cystourethrography for diagnosis of vesicoureteral reflux in children

Background:Vesicoureteral reflux is the most common urinary congenital anomaly in children.Given the risk associated with radiation exposure there has been an increasing need for radiation-free method in the diagnosis and follow-up of the vesicoureteral reflux.The aim of our study is to compare conventional urosonography without contrast enhancement and x-ray voiding cystourethrography.Patients and Methods:Children with recurrent urinary tract infection with suspected vesicoureteral reflux were included to the study.Vesicoureteral reflux is demonstrated and graded by x-ray voiding cystourethrography.DMSA is used for the evaluation of renal scar.Conventional sonographic procedure was performed in all patients.Ureterovesical junction insertion angle was evaluated.The diameter and length of the ureterovesical junction were also measured.Results:268 children enrolled to the study.Vesicoureteral reflux was demonstrated in 62 children by x-ray voiding cystourethrography.Ureterovesical junction insertion angle measurement had a statistically significant relation for right and left vesicoureteral reflux presence(right:r:.646,p:.01 and left:r:.446,p:.01).Diagnosis sensitivity of vesicoureteral reflux with conventional ultrasonography is 95.10%and specificity is 81%(Youden’s index 76.1%)for the cutoff value of the ureterovesical junction insertion angle is 28.6 degrees.Positive predictive value is 87.2%,negative predictive value is 94.73%and diagnostic accuracy is 86.29%with conventional ultrasonography.Conclusions:Measurement of ureterovesical junction insertion angle,length and diameter by conventional urosonography is an easy accessible and cheap technique with high sensitivity and specificity for the diagnosis and followup of the vesicoureteral reflux without exposure to ionizing radiation.

Analysis on clinical distribution and drug resistance of Klebsiella pneumoniae isolated for 5 consecutive years

Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatment for the infection of Klebsiella pneumoniae.Methods:1,192 strains of Klebsiella pneumoniae isolated from clinical specimens from 2012 to 2016 were collected.The strains were identified by VITEK-2 Compact Microbiological Identification System,and the corresponding results of the antimicrobial susceptibility test were interpreted in accordance with the standards recommended by Clinical and Laboratory Standards Institute(CLSI).Results:1,192 strains of Klebsiella pneumoniae were mainly isolated from sputum(65.6%),and most of them were from Respiratory Medicine Department and Medical Intensive Care Unit of Respiratory Medicine Department(MICU),accounting for 41.4%.Out of 1,192 strains,448 strains were detected to produce extended-spectrum beta-lactamases(ESBLs),accounting for 37.6%.In addition,the detection rates of ESBL-producing Klebsiella pneumoniae for 5 consecutive years showed an increasing trend year by year,and they were higher than the national average values published by China Antimicrobial Resistance Surveillance System(CARSS)in the corresponding period.The drug resistance rate of ESBL-producing Klebsiella pneumoniae was significantly higher than that of non ESBL-producing strains.Conclusions:The infection caused by Klebsiella pneumoniae mainly occurs in the lower respiratory tract,and the drug resistance rates of Klebsiella pneumoniae to antibiotics in the drug susceptibility spectrum are maintained at a high level.Therefore,the rational selection of antibiotics for the clinical treatment of lower respiratory tract infection caused by Klebsiella pneumoniae must be based on the production of ESBLs and the results of antimicrobial susceptibility test.

Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

Objective: To investigate the effect of silencing CD105 and Ki67 gene expression on OVCAR3 cell line in ovarian cancer. Methods: According to cell transfection results, OVCAR3 cells were divided into CD105-siRNA, Ki67-siRNA, CD105-siRNA+ Ki67-siRNA, negative control (NC1 and NC2) and control (non-transfection) groups. The effect of silencing CD105, Ki67 and their combined gene on proliferation, migration, invasion and apoptosis of human ovarian cancer cells can be studied and analyzed with MTT assay, wound-healing assay, Transwell chamber and flow cytometry. Results: Compared with their respective negative control and liposome control groups, proliferation, migration and invasion abilities of OVCAR3 cells were decreased obviously in CD105-siRNA, Ki67-siRNA and CD105-siRNA + Ki67-siRNA groups after gene silencing. Whereas, the most significant change was detected in CD105-siRNA + Ki67-siRNA group along with obviously increased apoptosis rate of cancer cells, and the differences were of statistical significance (p < .01, p < .05). Conclusions: Expression vectors of CD105-siRNA and Ki67-siRNA can inhibit the proliferation of OVCAR3 cells, reduce their abilities of migration and invasion, and induce apoptosis of tumor cells. The effect of combined gene silence will be more significant.

Expression of IL-6 and TNF-α in benign prostatic hyperplasia combined with histological inflammation

Objective:To detect the expression of TNF-αand IL-6 in simple benign prostatic hyperplasia(BPH)and BPH combined with prostatitis,and to evaluate the effect of inflammation of the development and progression of BPH.Methods:Specimens of 90 patients with BPH underwent transurethral resection of prostate(TURP)were obtained.All speci-mens were divided histologically by HE staining into group of 35 cases of simple BPH(A group)and group of 55 cases of BPH combined with prostatitis(B group).Proteins levels of TNF-αand IL-6 were examined by immunohistochemistry.All patients’clinical indicators including PSA,prostate volume,age,urinary flow rate were recorded,and all patients were diagnosed as BPH by pathology.Results:All patients of B group were found combined with chronic prostatitis.The constituent ratio was 61%.Patients’prostate volume and PSA of B group were significant higher than A group(p<.05),but there were no significant differences in urine flow rate and patients’age(p>.05).As compared with A group,the expression of TNF-αand IL-6 significantly increased in B group(p<.05).The results of analysis were as follows:there existed a moderate correlation between prostate volume and patients’age(r=0.430,p<.001);there existed a moderate correlation between prostate volume and the degree of inflammation(r=0.610 and r=0.609,p<.01);there existed a moderate correlation between PSA and the degree of inflammation(r=0.572 and r=0.487,p<.01);there was not significant correlation between PSA and patients’age(r=0.065,p>.1);controlling influence of the age factor by partial correlation analysis,there was still a significant correlation between the prostate volume and level of inflammation.Conclusions:Most BPH patients are combined with prostatitis especially chronic inflammation.TNF-αand IL-6 are two-pro-inflammation cytokines,which obtain high expression in the prostate tissue combined with histological inflammation.Patients’prostate volume and PSA of BPH combined with prostatitis are significantly higher than those of simple BPH.However,there are no significant differences in urine flow rate and patients’age.These two cytokines may play an important part in promoting BPH and secretion of PSA.

The application value of TCD combined with CDUS in the large artery atherosclerosis

Objective:To explore the value of TCD and CDUS on diagnosis of large artery atherosclerosis.Methods:In our study,90 patients who had been admitted to our hospital and diagnosed as LAA according to CISS typing were selected as the research object.A retrospective analysis was conducted,using CTA as golden standard for diagnoses of stenoses.Explore the risk factors of LAA,the reliability of TCD and CDUS in diagnosis of the stenosis and distribution of stenosis in each vessel.Results:Hypertension and type 2 diabetes are risk factors of LAA vascular stenosis.Middle cerebral artery stenosis detection by TCD carries the highest reliability(sensitivity 90.91%,specificity 97.89%,positive predictive value 96.15%,the negative predictive value was 94.9%,the positive likelihood ratio was 43.18,negative likelihood ratio 0.09,Kappa=0.9).The consistency of TCD is as good as CTA in head and neck part.The negative coincidence rate was higher in the anterior circulation vessels(97.98%),followed by the coincidence rate of severe stenosis(95.23%).However,the detection rate of posterior circulation vessels positive,moderate and severe stenosis,mild stenosis gradually decreased by TCD.CDUS carries general reliability in detecting intracranial extracranial stenosis,the highest reliability in internal carotid artery(kappa 0.82),and the worst consistency in vertebral artery(kappa 0.38).The negative coincidence rate of CDUS in extracranial cerebrovascular stenosis was highest(97.22),followed by the overall compliance rate(91.55%).242 branches of vascular stenosis were discovered in our study,among which intracranial artery stenosis 61.15%(148/242)is higher than that in extracranial artery 38.85%(90/242),ratio of moderate to severe stenosis is as high as 82.23%(199/242).Intracranial artery stenosis in ICA is extremely common(51.11%,46/90),and MCA is the common area of intracranial artery stenosis(37.16%,55/148).Conclusions:(1)Hypertension,type 2 diabetes are risk factors for LAA stenosis.(2)TCD detection of intracranial vascular stenosis carries the best consistency,and reliability of MCA is the highest.In our search,MCA possesses the highest rate of stenosis,and negative coincidence rate by TCD examination was the highest,which could be used for screening method for stenosis.The reliability of TCD in diagnosis of moderate and severe stenosis was relatively better than mild stenosis.(3)CDUS detection of anterior circulation lesions is better than that of posterior circulation lesions.Evaluate effect on moderate and severe stenosis was better than that of mild one.The ratio of ICA stenosis was the highest,but the effect on VA was poor.(4)The incidence of intracranial vascular stenosis is more common than that of the intracranial vascular stenosis.

Animal study on CD4^(+) CD25^(+) regulatory T cells for treating female mouse with recurrent spontaneous abortion

Objective:To explore immunotherapy effectiveness of the CD4^(+)CD25^(+) regulatory T cells for treating female mouse with recurrent spontaneous abortion(RSA)by animal experiments.Methods:Mononuclear lymphocytes were isolated from the blood(instead of cord blood)of new-born baby of KunMing Bai mouse or BALB/c male mouse with normal birth ability(as unrelated third party blood source)by density gradient centrifuga-tion method.The CD4^(+)CD25^(+) regulatory T cells were selected by magnetic-activated cell sorting from mononuclear cells of cord blood cells.CBA/J female mouse copulated with DBA/2J male mouse was utilized as RSA animal model.Pregnant RSA mice were injected different types of lymphocytes through tail vein.Independent sample t-test was used to analyze the data from each group.Results:The proportion of CD4^(+)CD25^(+)T cells in CD4^(+)T cells was(17.49±0.60)%in CD4^(+)CD25^(+) regulatory T cells injection group,which was statistical significant higher than that of mononuclear lymphocyte injection group(14.68±0.83)%,sterile PBS group(9.54±0.85)%or no injection group(9.28±0.68)%(p<.05,t-value was 4.754,13.242 and 15.621,respec-tively).The Foxp3 relative protein expression level of CD4^(+)CD25^(+) regulatory T cells injected group was 5.85±0.45,which was also significant higher than that of mononuclear lymphocyte injection(2.86±0.54),sterile PBS group(1.08±0.16)or no injection group(1.00±0.00)(p<.05,t-value was 7.276,17.227 and 18.635,respectively).Finally,two times of CD4^(+)CD25^(+)T cell injected group at the 4 th and 8 th day had well effect for RSA mouse,and embryo sorption rate was(4.92±0.08)%,which significant lower than that of two times of mononuclear lymphocyte injected group(13.07±0.06)%,sterile PBS group(23.11±0.12)%,or no injection group(25.47±0.11)%(p<.05,t-value was-2.603,-4.012 and-4.700,respectively).Conclusions:Pregnant mouse with RSA injected CD4^(+)CD25^(+)T cells several times for immunotherapy can get better effec-tiveness than that of pregnant mouse injected traditional mononuclear cells.

Generalized lymphadenopathy as an atypical initial clinical manifestation in rheumatoid arthritis and a possible hypothetical mechanism

Objective:To describe a 60-year-old male with diffuse generalized lymphadenopathy preceding a diagnosis of rheumatoid arthritis.Case presentation:The patient was admitted on suspicion of lymphoma.Chest positron emission/computed tomography showed enlarged lymph nodes bilaterally,mediastinum in the perihilar,retroperitoneum,and inguinal regions,with normal hematopoiesis of bone marrow.Lymph node biopsy revealed reactive follicular lymphadenopathy.Polyarthritis was present,and a rheumatoid factor test was positive eight months after the initial medical evaluation.A diagnosis of rheumatoid arthritis associated with generalized lymph node involvement was made.The patient was treated with leflunomide and corticosteroids and showed complete recovery without recurrence at 18 months of follow-up.Conclusions:Once histological findings of reactive lymph node hyperplasia are established as primarily related to rheumatic disease activity,clinicians should consider a possible diagnosis of rheumatoid arthritis.

Culturally tailored DSMES:A new key for South Asian patients with Type 2 Diabetes Mellitus

South Asians have an exceptionally high risk for developing Type 2 diabetes mellitus.It is very challenging for healthcare providers to successfully manage diabetes and control glucose levels at target due to the unique lifestyle of the South Asian population.Culturally tailored diabetes self-management education and support(DSMES)can be more effective in guiding South Asian patients with Type 2 diabetes.Unique considerations to address lifestyle modification for South Asians include a diet that typically consists of a high carbohydrate to lipids/proteins ratio,preference for high glucose index fruits,regular intake of traditional sweets or desserts,late afternoon tea break followed by late dinner,lack of vigorous exercise(yoga or walking being the preferred activity),lack of DSMES knowledge and skills,and poor access to culturally appropriate resources for diabetes care.We present a 38-year-old male diagnosed with diabetes four years ago who showed poor glucose control before our intervention.Our interventions included education on the importance of blood glucose monitoring,exercise,and diet.Based on our experience with this case,we propose the following recommendations for a tailored approach to DSMES for South Asian patients with Type 2 diabetes:make appropriate dietary changes(decrease total daily caloric intake,decrease the percentage of carbohydrates,add low glucose index fruits and vegetables,avoid late afternoon tea breaks,eat dinner before 8 PM);incorporate appropriate daily physical activity;and monitor blood glucose daily for prompt feedback.